Methods including quantification of rna species, Biology

1.   Northern blotting

The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.

In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.

2.    Reverse transcriptase-real time quantitative pcr

The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.

3.   Hybridisation micro array

This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.

4.  Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.

 

Posted Date: 3/6/2013 6:05:20 AM | Location : United States







Related Discussions:- Methods including quantification of rna species, Assignment Help, Ask Question on Methods including quantification of rna species, Get Answer, Expert's Help, Methods including quantification of rna species Discussions

Write discussion on Methods including quantification of rna species
Your posts are moderated
Related Questions
can u show the different activities on the topic

why is it necessary to grind the food samples before testing?

Q. Identification and Care of Taxonomic collections? Collection of plant specimens is essential for taxconomic research. Herbarium specimens become a permanent record. Select t

what are the biological significance of the skeleton?

Q. What is QT Dispersion? Is the difference between the QT interval measured from one part of the heart and the QT interval measured from another part of the heart. The QT inte

Quantitative Methods - Therapeutic diets Quantitative Methods: These are often essential for constructing  Lherapeutic diets.  The  two ways by which  this could be done are as

Dracunculiasis (guineaworm infestation) Dracunculiasis, a disease of man, which has been known since antiquity, is caused by the nematode parasite Dracunculus medinesis. The p

Atherosclerosis, the most common part of hardening of the arteries, is characterized by the presence of cholesterol-rich arterial thickenings (atheromas).  This progressive  diseas

How to evaluate blanching efficiency? Finally, let us learn how to evaluate blanching efficiency? Normally, two of the more heat resistant plant enzymes, namely peroxidase and