Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
1. Northern blotting
The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.
In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.
2. Reverse transcriptase-real time quantitative pcr
The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.
3. Hybridisation micro array
This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.
4. Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.
Weight of Plants - Quantitative Characters Weight is one of the most important quantitative characteristics of plants. Quantitatively growth is best measured on the basis of d
COMMON DISEASE OF SHEEP - 1 . ANTHRAX - Persons working in wool factories may get infection by breathing. 2 . BLACK QURTER 3 . HAEMORRHAGIC S
SKIN CANCER: CAUSES AND TREATMENT PURPOSE: The objective of this assignment is to make you aware of the structure and function of your skin, what are the dangers that your skin ma
How do the products of meiosis I differ from those of meiosis II? In meiosis I, the offspring cells are haploid but every cell contains two copies of the chromosome because th
Theoretically you can build a light microscope with unlimited magnification, but can you build a light microscope with unlimited resolution? Why or why not? (Hint: what really cont
Q. Determine ST-Segment Elevation? Ans. Exercise induced ST-segment elevation is common in subjects with post MI stress tests in leads where Q-waves are present. It has be
Phosphorus deficiency Deficiency of phosphorus is usually primary and is widespread under natural conditions. It is characterized by pica, poor growth, infertility, and osteody
Explain Osmotic Pressure Osmosis, as you may already know, refers to the flow of solvent into a solution, or from a more dilute solution to a more concentrated solution, when t
Define about the Ultraviolet rays - carcinogenic? Ultraviolet rays: There is ample evidence from epidemiological studies that ultra violet rays derived from the sun induce an i
Quantitative Characters - Population Density Density denotes the average number of individuals of a particular species in a unit area. In other words, it represents the numeri
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd