Methods including quantification of rna species, Biology

1.   Northern blotting

The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.

In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.

2.    Reverse transcriptase-real time quantitative pcr

The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.

3.   Hybridisation micro array

This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.

4.  Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.

 

Posted Date: 3/6/2013 6:05:20 AM | Location : United States







Related Discussions:- Methods including quantification of rna species, Assignment Help, Ask Question on Methods including quantification of rna species, Get Answer, Expert's Help, Methods including quantification of rna species Discussions

Write discussion on Methods including quantification of rna species
Your posts are moderated
Related Questions
Explain Dietetics Dietetics  has been defined as  the science and art of feeding individuals based  on the principles of  nutrition. It can also be  said  to  be  the "science

Classes of Life Form The five life form classes are: Phanerophytes, Chamaephytes, Hemicryptophytes, Cryptophytes, and Therophytes.

Define the Interaction of Vitamin C with other Nutrients? Having gone through the discussion above it must be evident to you that nutrients are interdependent and are related t

Explain what is Molds ? Zygomycota, or molds, are a type of fungus made of filamentous hyphae that grow on decomposing material. The molds actually aid in the decomposition pro

Neurochemistry of Attention Different neurotransmitters systems are implicated in attention. Cholinergic, dopaminergic and serotonergic systems play major roles in neurochemica

what are the organs of respiration in lower forms of animals?

What isthe Riccia

Relate the oxide layer and biocompatibility of titanium. The compatibility of a metal with its host environment depends on its resistance to biodegradation and on the degree of

Blastomycosis Blastomyces dermatidis is a dimorphic fungus existing as a mold in the soil (saprophytic stage) and as a yeast in tissues (parasitic stage). It causes systemic fu

The major component of food is protein. The proteins ingested as part of our diet are not the same proteins required by the body, nor can large molecules be absorbed from the gut.