Isolation of dna fragments, Biology

Isolation of dna fragments bound strongly with protein

1.     Through pcr

Pcr permits isolation of dna fragments from genomic dna through selective amplification of a specific area of dna. Large amount of pure dna fragments bound by protiens can be seperated.

2.     Chromatin immuno precipitation

This technique gives one way of empirically finding the sites of dna that a known gene regulatory protein occupies under specific set of conditions. In this technique proteins are covalently attached to dna to be isolated, dna is mechanically sheared into minute fragments. Antibody directed against a known regulatory protein is then utilized to purify dna which is covalently attached to the protein. This dna fragments are hybridized to micro arrays that comprises of the complete genome displayed a series of dna fragments, the precised genomic location of every dna fragment can be found.


Posted Date: 3/6/2013 6:09:51 AM | Location : United States

Related Discussions:- Isolation of dna fragments, Assignment Help, Ask Question on Isolation of dna fragments, Get Answer, Expert's Help, Isolation of dna fragments Discussions

Write discussion on Isolation of dna fragments
Your posts are moderated
Related Questions
Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Water Soluble Vitamins B-vitamins are abundant in milk and other feeds. B-vitamins are synthesized by rumen microorganisms, beginning soon after a young animal begins feeding.

Explain about Food processing and engineering? Fundamental engineering concepts like momentum, heat and mass-transport systems; engineering aspects of food processing plant ope

Define briefly about the Cereal Proteins? Cereal grains, properly ripened and dried for optimum storage stability, have protein content ranging from 6-20%. Proteins are found i

whats the difference in respiratory system in mammals,reptilia and amphibian?

what are enzymes?

Q. Can you explain Metallized films? In metallized films, plastic films such as those of polyester, polyamide and polypropylene are coated with aluminium to enhance the barri

Kidney Disease   The target BP in chronic kidney disease is set at 130/80.  This will retard the progression of renal function. The drugs recommended are the ACEI/ARBs. These c

A Collective Study performed on Asian Chinese Normotensive Population to Obtain Healthy Normal Range of some Waveform Indices  Background-  Arterial blood pressure waveforms c

Explain Procedure for Separation of Amino Acids by Paper Chromatography? Now carry out the procedure following the steps given herewith: 1. Take a circular Whatman No 1 filt