Isolation of dna fragments, Biology

Assignment Help:

Isolation of dna fragments bound strongly with protein

1.     Through pcr

Pcr permits isolation of dna fragments from genomic dna through selective amplification of a specific area of dna. Large amount of pure dna fragments bound by protiens can be seperated.

2.     Chromatin immuno precipitation

This technique gives one way of empirically finding the sites of dna that a known gene regulatory protein occupies under specific set of conditions. In this technique proteins are covalently attached to dna to be isolated, dna is mechanically sheared into minute fragments. Antibody directed against a known regulatory protein is then utilized to purify dna which is covalently attached to the protein. This dna fragments are hybridized to micro arrays that comprises of the complete genome displayed a series of dna fragments, the precised genomic location of every dna fragment can be found.

 


Related Discussions:- Isolation of dna fragments

Determine amount of intracellular potassium ions in neuron a, Determine amo...

Determine amount of intracellular potassium ions in neuron A Neuron A is a healthy neuron with all the usual ion channels.  When at rest with a membrane voltage of R millivolts

What is short acting insulin, Q. What is Short acting insulin? Short ac...

Q. What is Short acting insulin? Short acting: This type of insulin begins working quickly, works hardest 2- 3 hours after injection but is completely gone after 4-6 hrs. So if

List disbeliefs which patients have about insulin, Q. List disbeliefs which...

Q. List disbeliefs which patients have about insulin? a) Insulin is not effective. Some patients believe that insulin is not effective for treating diabetes. b) Insulin c

Planning of nursing care - hypospadias, Planning of Nursing Care Planni...

Planning of Nursing Care Planning of the nursing care depends on the decision of the surgery. The surgery may be performed in two stages depending upon the degree of the d

Guanine, Guanine is one of the nitrogenous bases in the nucleic acids, gua...

Guanine is one of the nitrogenous bases in the nucleic acids, guanine is one of the two purine bases.

Structure of phloem, Structure of Phloem In this we will explain the s...

Structure of Phloem In this we will explain the structural and functional relationship of phloem and compare it with xylem. The location of phloem with respect to other tissue

Define the effect of breastfeeding on mother, Normal 0 false ...

Normal 0 false false false EN-IN X-NONE X-NONE

Types of ribosomes, TYPE S OF RIBOSOMES Ribosomes are of two types: - ...

TYPE S OF RIBOSOMES Ribosomes are of two types: - 1 . Procaryotic Ribosomes - 70 s = Occur in cytoplasm of prokaryotes, and also in mitochondria, and Chloroplast o

Do sponges have circulatory system, Q. Do sponges have circulatory, nervous...

Q. Do sponges have circulatory, nervous and excretory systems? Sponges do not have a nervous system neither excretory system nor circulatory system.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd