Isolation of dna fragments, Biology

Isolation of dna fragments bound strongly with protein

1.     Through pcr

Pcr permits isolation of dna fragments from genomic dna through selective amplification of a specific area of dna. Large amount of pure dna fragments bound by protiens can be seperated.

2.     Chromatin immuno precipitation

This technique gives one way of empirically finding the sites of dna that a known gene regulatory protein occupies under specific set of conditions. In this technique proteins are covalently attached to dna to be isolated, dna is mechanically sheared into minute fragments. Antibody directed against a known regulatory protein is then utilized to purify dna which is covalently attached to the protein. This dna fragments are hybridized to micro arrays that comprises of the complete genome displayed a series of dna fragments, the precised genomic location of every dna fragment can be found.

 

Posted Date: 3/6/2013 6:09:51 AM | Location : United States







Related Discussions:- Isolation of dna fragments, Assignment Help, Ask Question on Isolation of dna fragments, Get Answer, Expert's Help, Isolation of dna fragments Discussions

Write discussion on Isolation of dna fragments
Your posts are moderated
Related Questions
Retina As invagination of the optic vesicle carries on to form the double walled optic cup the two layers of the optic cup start to differentiate in different directions. Pigm

Define essentiality of Sulphur for growth of micro-organism? Sulphur is a part of some amino acids, some carbohydrates, biotin and thiamine. It is obtained either as elemental

For many of the mammalian Hox genes, it has been possible to determine that some of them are more similar to one of the insect HOM-C genes than to the others. Describe an experimen


What are two things that enter the cell for cellular respiration? Carbohydrate molecules and oxygen enter the cell during cellular respiration.

filter feeding mode of nutrition is observed in- a) amoeba b) euglena c)paramoecium d) all of the above

what is the necessity of interdependence of a volvox organism in the colonial existence?

Define Precautions for Check the Presence of Rhodamine B? 1. Sample should be clean and dry. 2. Dispose off the paraffin oil carefully, so that it does not contaminate food/

Define the role of riboflavin in Drug and lipid metabolism? Flavoproteins catalyze dehydrogenation reactions, as well as, hydroxylations, oxidative decarobxylations, deoxygenat

State in brief about the Fungi It is heterotrophic plants, larger than the bacteria. Those that live on the dead tissues of organic substances are called saprophytic. They pla