Gateway reaction-entry clone and destination vector, Biology

Assignment Help:

1530_Gateway Recombination reaction.png

(1) From the above gateway reaction (entry clone and destination vector);

(i) which plasmid will be selected for when transformed into E.coli(A or B) and why?

(ii) which antibiotic would you included in the selection?

(iii) which strain of E. coli would you transform the gateway reaction into?

(iv) which strain of E. coli do you need to maintain and amplify the entry clone

(v) which strain of E. coli do you need to maintain and amplify the destination vector.


Related Discussions:- Gateway reaction-entry clone and destination vector

Cuticle, what does cuticle mean

what does cuticle mean

Larval forms - porifera, Larval forms - Porifera In the majority of sp...

Larval forms - Porifera In the majority of sponges the larval stage develops in the body of the parent. The larva is generally at the blastula stage of development. Figure dis

How is gas exchange done in flatworms, How is gas exchange done in flatworm...

How is gas exchange done in flatworms? Platyhelminthes exchange gases exclusively by diffusion by their body surface. This is only possible because all cells are localized rela

Discuss fatty acid with free radical, Discuss fatty acid with free radical ...

Discuss fatty acid with free radical The hydrogen is removed from the fatty acid chain to yield a free radical. The removal of hydrogen takes place at the carbon atom next to t

Which are growth tissues of plants, Which are growth tissues of plants? How...

Which are growth tissues of plants? How do they categorize and where can they be found? Growth tissues of the plants are the meristems. The Meristems are the tissues that produ

Radial and biradial - metazoa, Radial and Biradial-Metazoa Radial sym...

Radial and Biradial-Metazoa Radial symmetry is the symmetry in which the parts are so arranged around a central axis or shaft, like the spokes of a wheel, that any vertical c

Explain the stages in taxonomic procedures - alpha taxonomy, Explain the St...

Explain the Stages In Taxonomic Procedures? Divided into three levels or phases: 1) Alpha (α) phase, 2) Beta (β) phase, 3) Gamma (γ) phase. Alpha Taxonomy The

Water movement through the integument, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd