(1) From the above gateway reaction (entry clone and destination vector);
(i) which plasmid will be selected for when transformed into E.coli(A or B) and why?
(ii) which antibiotic would you included in the selection?
(iii) which strain of E. coli would you transform the gateway reaction into?
(iv) which strain of E. coli do you need to maintain and amplify the entry clone
(v) which strain of E. coli do you need to maintain and amplify the destination vector.