Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What are the complementary base-pairing rules for biology, What are the com...

What are the complementary base-pairing rules for biology? In DNA, Adenine bonds with Thymine, Cytosine bonds with Guanine. In RNA, Thymine is changed with Uracil (bases capita

Amphibia, describe general characters and classification of amphibia in bri...

describe general characters and classification of amphibia in brief

Can you explain antibiotics, Q. Can you explain Antibiotics? The antibi...

Q. Can you explain Antibiotics? The antibiotics are the chemicals produced by microorganisms which are able to destroy or inhibit the growth of other microorganisms. The therap

How will this increase survival, Why would we evolve so that when we are st...

Why would we evolve so that when we are stressed our immune system is suppressed? How would this increase survival?

Mammalian heart, Mammalian Heart The division of heart and separation ...

Mammalian Heart The division of heart and separation of systemic and pulmonary circulation is complete in birds and mammals. The structure of mammalian heart and also how the

TISSUES, difference between axon and cyton

difference between axon and cyton

#title.growth hormone., according to the graph,which age is the amount og g...

according to the graph,which age is the amount og growth hormone produced at its highest?

What are the poriferans, Q. What are the poriferans? The phyla Porifera...

Q. What are the poriferans? The phyla Porifera include the simplest creatures of the animal kingdom. Sponges are aquatic sessile beings they are not able to move by themselves

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd