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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain about Hyperglycemia? It is a Greek term: hyper -meaning excessive; glyc- meaning sweet; and emia- means "of the blood". It is a condition in which an excessive amoun
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The Social Environment When the child is admitted to the hospital his social environment is totally changed and all health team members are strangers for him. It is your resp
Q. What is the relationship between enzyme and vitamins cofactors? Many vitamins are enzyme cofactors that cannot be synthesized by the organism and must be acquired from the d
Why do membranes figure so prominently in eukaryotic cells? What essential function do they serve?
OPEN HEART SURGERY : When patient is connected lo cardio pulmonary bypass for an operative procedure, it is considered to be open-heart operation. In a routine coronary artery b
Q. Explain the Spring clip model? The spring clip model differs from the spring model in a way that the force applied is comparatively slower. This also depicts elasticity or p
Q. Do the phosphate and the pentose groups give heterogeneity or homogeneity to the nucleic acid chains? What about the nitrogen- containing groups? Supported by that, which of tho
To show that growing seeds take in oxygen Cork up one end of a tube, having first placed inside some damp cotton wool and some mustard seeds. Immerse the open end in dilute ca
Organisation of Nervous System Nervous systems are composed of nerve cells or neurons and glial cells. In the latter half of the 19 th century it was strongly believed that
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