Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is vacuole , What is Vacuole ? Another structure found only in pla...

What is Vacuole ? Another structure found only in plant cells is the large central vacuole. The vacuole stores enzymes and waste products, in addition to providing the turgor p

Growth and development, what is the clear explanation of growth and develop...

what is the clear explanation of growth and development

Define about the metabolism of copper, Define about the Metabolism of Coppe...

Define about the Metabolism of Copper? In food, most copper is present as Cu 2+ and some as Cu 1+ . This copper is bound to organic compounds especially protein. Gastric HCI p

Hemocytometer, A device called a hemocytometer is often used to estimate th...

A device called a hemocytometer is often used to estimate the number of cells in a culture. Cells are suspended in fluid. A small volume of the fluid is placed into a special chamb

Respiratory system in fishes, The fishes belong to class Pisces under verte...

The fishes belong to class Pisces under vertebrates. 2. In fishes, the respiratory system consists of mouth, pharynx, internal branchial apertures, branchial pouches and external b

What substance is the acidic flavor of fermented milk due, Q. To what subst...

Q. To what substance is the acidic flavor of fermented milk due? Some bacteria ferment milk lactose by lactic fermentation producing lactic acid this product is responsible for

Diet for cardiac patients, Diet Continue diet as prescribed at hospita...

Diet Continue diet as prescribed at hospital. Some may continue fluid and salt restriction, some may have to restrict iron and Vitamin K rich food stuff. The patient needs t

Neuroglia - organisation of nervous system, Neuroglia - Organisation of Ner...

Neuroglia - Organisation of Nervous System We may refer neuroglia as the connective tissue of the nervous system. It involves all elements of the nervous system other than ne

What is pleiotropy, What is pleiotropy? Pleiotropy (or pliotropy) is th...

What is pleiotropy? Pleiotropy (or pliotropy) is the occurrence in which a single gene conditions several dissimilar phenotypical traits. Some phenotypical traits might be s

Relate fibro-osseous retention and osseointegration, Fibro-osseous Retenti...

Fibro-osseous Retention V/S Osseointegration Fibro-osseous retention is the tissue to implant contact interposition of the healthy, dense collagenous tissue between the implant

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd