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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Oxidation and Reduction? Oxidation and Reduction : Most people are familiar with oxidation as it pertains to the rusting of metals. Oxygen grabs electrons from iron
S n ake bite Biting by a poisonous snake is manifested by clinical findings of local swelling and nervous symptoms. E t iology: The poisonous snakes have different typ
Biodynamic: This dealing with energetic of organisms. Biodynamic is a method of organic farming which treats farms as unified or individual organisms, emphasizing balancing the ho
what are peptides and poly peptides hormones
a) Why do C 4 plants have dimorphic chloroplasts? Describe the different steps involved in C 4 photosynthetic carbon cycle in such plants.
How is the genetic determination of sex established in humans? In the diploid genome of the human beings, there are 46 chromosomes, 44 of them are autosomes and two are sex chr
Q. What are the Symptoms of pectic ulcer? Increased gastric tone and painful hunger contraction when stomach is empty. Hunger contraction 1-3 hours after meals is the main com
Results : In critical pulmonary stenosis in infancy, surgical mortality is 6 per cent. For children and adults with isolated pulmonary valve stenosis, the mortality approaches 0
Japanese encephalitis Japanese encephalitis (JE) is a mosquito-borne encephalitis and is caused by flavivirus belonged to the family Flaviviridae. It is a zoonotic disease, i
Steps of Research Process: Identify the Problem: The first task in the planning stage is to identify and stage the problem, as you have already learnt in the earlier
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