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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Interact with the Parent and Child to Exchange Information At this time the nurse can learn why the child has come to the hospital and the concerns and expectations he and hi
What will happen to resting membrane potential if potassium succinate were injected into the intracellular environment (cytoplasm) of a neuron?
How is the genetic determination of sex established in humans? In the diploid genome of human beings there are 46 chromosomes, 44 of them are autosomes and two are sex chromoso
how twins are produced and why
Explain Standard titration - Estimation of Vitamin C in Lemon Juice? Pipette 5 ml of standard ascorbic acid solution into a 100 ml conical flask. Fill the burette with the dye s
Which Foods allowed in soft diet:- Soups - mildly flavoured - broths and cream soups. Beverages - all Meat - moist, tender meat, fish or chicken, cottage cheese, eggs
At the end of the ventricular contraction, maximum arterial pressure is attained and blood flow is most rapid. This is correctly termed the: a) Arterial maximum b) Diastolic
what is phylum mollusca?
Temperate rain forests - Ecosystem The temperate rain Forests are colder than any other rain forest and exhibit a marked seasonality with regard to temperature and rainfall. R
what are the biotechnology techniques used in the fisheries?
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