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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
1. Explain how bats determine the distance (range) of their target using echolocation. Be sure to describe the properties of their call that make it possible (use a sketch), and ho
Define Nutritional Management of Food Allergies and Food Intolerance? The term "hypersensitivity" is general and may include true allergies, reactions that do not affect the im
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Describe the Phylum Echinodermata in details? The Phylum Echinodermata takes its name from two words of Greek origin: "echino," ; which means "prickle" or "spine," and "derma,"
The aqueous solution with the lowest pH is: Select one: a. 0.01 M HCl. b. 0.1 M acetic acid (pKa = 4.86). c. 0.1 M formic acid (pKa = 3.75). d. 0.1 M HCl. e. 10 t
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Define Stage - Parts of the Microscope? It is a fixed platform positioned about halfway up the arm and provide surface for keeping the slide. Slide is held in position either b
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Legal level -Pollution control Pollution must be controlled by effective legislation also. Like USA, Japan, Germany and many other countries a comprehensive legislation for pr
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