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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What does S-shaped pattern of population growth show? How is J-shaped pattern dissimilar from it and why?
#questionThree actographs (look closely to see the labels A, B, and C). Assume that the abscissa is either 24 or 48 hours for each actograph. You are asked to write two sentences f
ways in which modern agricultural activities may threaten survival species
Do all vascular plants develop annual rings? Vascular plants are those, which have phloem and xylem structures within them to transport water and nutrients around the plant. Mo
how to determine that the phenotype of a wild type is due to the action of one or two genes?
Determine the Food Sources of Iodine? Please note that unlike other minerals studied so far, like selenium, the iodine concentration in foods is highly variable and also depend
Advantages of Autonomous Transactions An autonomous transaction, once started is fully independent. It shares no locks, resources, or commit-dependency with the main transacti
#what is adaptation?
How is snow - blindness caused in humans? a) Mention the site where syngamy happens in amphibians and reptiles correspondingly.
This innovative, pacemaker-based approach to the treatment of patients with heart failure who have a wide QRS complex (>140 ms) on 12-lead ECG aims at providing electromechanical c
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