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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the complementary base-pairing rules for biology? In DNA, Adenine bonds with Thymine, Cytosine bonds with Guanine. In RNA, Thymine is changed with Uracil (bases capita
middle man of the body
describe general characters and classification of amphibia in brief
Q. Can you explain Antibiotics? The antibiotics are the chemicals produced by microorganisms which are able to destroy or inhibit the growth of other microorganisms. The therap
Why would we evolve so that when we are stressed our immune system is suppressed? How would this increase survival?
Classification of plants and animals
Mammalian Heart The division of heart and separation of systemic and pulmonary circulation is complete in birds and mammals. The structure of mammalian heart and also how the
difference between axon and cyton
according to the graph,which age is the amount og growth hormone produced at its highest?
Q. What are the poriferans? The phyla Porifera include the simplest creatures of the animal kingdom. Sponges are aquatic sessile beings they are not able to move by themselves
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