Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Left border of the heart, Connect point A and D by line which is convex to ...

Connect point A and D by line which is convex to the left. The left border is formed by the left ventricle except at its upper end which is formed by the left auricle where there i

What is the official name of pyruvic acid, Q. What is the official name of ...

Q. What is the official name of pyruvic acid? Pyruvic acid is named as 2-oxopropanoic acid it is thus a molecule made of three linearly bound carbons with one extremity forming

Air pollution - environmental pollution, Air Pollution - Environmental Poll...

Air Pollution - Environmental Pollution The threat due to air pollution became apparent only when some severe episodes caused human casualty in USA, Britain (London) and Japan

Determine analyses provided by study of human age pyramids, What are the an...

What are the analyses provided by the study of human age pyramids? The study of human age pyramids can give the following analyses: proportion of individuals at an economically

Define the history of food microbiology, Normal 0 false false...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Define aerobic exercise or aerobic energy system, Define Aerobic Exercise o...

Define Aerobic Exercise or Aerobic Energy System? To produce necessary energy, the body uses an aerobic pathway and an anaerobic pathway. Exercise that relies heavily on oxygen

Explain the concept of selective breeding, Explain the  concept  of  selec...

Explain the  concept  of  selective  breeding a) Understood  the  concept  of  selective  breeding  and/or  were  able  to  use  their Knowledge  of  Mendelian  genetics  to  e

Would you expect the muscle fibers of the tongue, Would you expect the musc...

Would you expect the muscle fibers of the tongue to be striated or smooth? What about the muscle of the diaphragm/ Explain your answer.

Muscular tissue, MUSCULA R TISSUE Muscular tissue in general develop...

MUSCULA R TISSUE Muscular tissue in general develops from the mesoderm of the embryo but the muscles of the iris of the eye and myoepithelial cells of the salivary, mammar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd