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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Definition and applications
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Parthenogenesis The diploid egg produced in the embryo sacs during diplospory and apospory develops into an embryo without fertilization, thus maintaining the sporophytic lev
Hydrarch - Kinds of Succession When succession takes place in a wet area, that is, succession progresses from hydric to mesic conditions. This can be further subdivided as:
Q. What are the Symptoms of gastritis? Anorexia, epigastric discomfort, heartburn and severe vomiting, pain in upper abdomen, Headache and even bleeding.
T ub e rc u l o si s Tuberculosis in animal is caused by Mycobacterium bovis , M. tuberculosis and M. avium and among these the most important causal agent is M.
classify cobra
how do honey bees breathe
1. Given a log phase bacterial culture with 1 x 106 cells per ml and a generation time of 20 minutes, how long does it take the culture to reach a density of 6.4 ? 107 cells per ml
How many distinct hexapeptides can be made from 20 amino acids?
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