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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How are roots affected by water? Grow some seedlings in single end of a glass dish or pan. When they are about 5 cm tall start watering them on one side only and a little dista
What is Electron Cloud Model ? Electron Cloud Model : In recent models of the atom, electrons form electron clouds, in which electrons travel in spherical and dumbbell-shap
Which of the following is a true statement regarding the Eyes Absent Protein? A. EYA is a transcriptional activator that having both a DNA binding domain and a transactivation
Explain the Theory or Principle for Determination of Fungal and Yeast Count? Fungi are widespread and present on food, equipments and processing and storage facilities. These a
THEORY OF ORGANIC EVOLUTION - LAMARCKISM Theory of the inheritance of acquired characters or Lamarckism put forwarded by Jean Baptist de Lamarck (1809) in his book "
Explain Theory of Evolution through Natural Selection The third theory of evolution through natural selection was put forward jointly by Darwin, and Wallace in 1859. This theor
Respiratory system of Crustacea ?
State the goals of Neuropsychological assessment Neuropsychological assessment therefore has twin goals. i) The first goal is to recognize the disrupted psychological compo
Use of Alternate Metabolic Pathways Plants surviving under water-logged conditions offer the simplest example of such a response. The submerged parts of such plants use the an
How Proteins can be Classified? I. Shape and size: fibrous proteins and globular proteins. Fibrous proteins play structural roles in organisms. Globular proteins consist of lo
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