Explain the Precautions for Sub-Culturing of a Culture

1. Sterilization of inoculating wire before and after the transfer is a must. Sterilized wire should not be kept on laboratory bench.

2. Never place the closure of tubes or lid of the plates on working bench or touch it with any surface.

3. Neck of the tubes should be flamed before and after the inoculation to avoid contamination.

4. Culture transfer should be done near the flame.

5. Do not dip the needle into the agar while inoculating slant or agar plate.

6. Never leave the culture tube open any longer than the amount of time needed to transfer the culture.