Explain lane eynon method procedure, Biology

Assignment Help:

Q. Explain Lane Eynon Method procedure?

You will be carrying out the procedure in two steps using Lane Eynon Method.

Step 1

It involves the standardization of copper sulphate solution. The procedure for this standardization can be conducted in the underlined manner as:

1) Pipette accurately 5 ml of Solution A and Solution B in conical flask of 250 ml capacity.

2) Heat this mixture to boiling on an asbestos gauge and add standard invert sugar solution from the burette about 1ml less than the expected volume which will reduce the Fehling solution of say, 48 ml.

3) Add 1 ml methylene blue indicator.

4) Carry out the titration and complete it within 3 minutes.

5) The change in blue to reddish brown colour due to cuprous oxide formation is taken as the end point.

6) From the volume of the invert sugar solution used, the strength of CuSO4 is calculated by multiplying the titrated value with 0.001 (mg/ml of the standard invert sugar solution). This is known as Fehling factor.

Note: Carry out the titration till you get consecutive titre value. This means you may have to repeat the titration 3-4 times till you get the same result.

Step 2

It involves the titration of the sample honey solution wherein the steps can be followed as given below:

1) Place accurately 1 gm of honey solution in 250 ml of volumetric flask.

2) Dilute with about 150 ml of water.

3) Mix thoroughly contents of glass and make the volume to 250 ml.

4) In another conical flask, add 5 ml of Fehling A + 5 ml of Fehling B.

5) Heat to boiling with 20 ml of water.

6) From burette add honey solution (approx 40 ml) and boil.

7) Add methylene blue indicator do the titration within 3 minutes.

8) Carry out the titration till blue colour changes to red.


Related Discussions:- Explain lane eynon method procedure

Which are the specialized conductive tissues of the plants, Which are the s...

Which are the specialized conductive tissues of the plants? The vascular tissues of the plants are the xylem and the phloem. Xylem is the plant tissue that forms the vessels t

Ciliary and flagellar movements, Normal 0 false false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain the character of pulse, Explain the Character of Pulse ? A norm...

Explain the Character of Pulse ? A normal pulse wave has an upstroke and down stroke. Dicrotic notch present on direct arterial traces is impalpable. Figure: Arterial

Define surface properties of proteins, Define Surface properties of Protein...

Define Surface properties of Proteins? The surface properties relates primarily to surface tension, emulsification and foaming characteristics of proteins, which are discussed

Explain about the phosphorus - macro minerals, Explain about the Phosphorus...

Explain about the Phosphorus - Macro Minerals? Phosphorus is the second most abundant element in the human body, comprising 30% of the total mineral content. An adult human bod

How many g of h2so4 does contain, How many g of H2SO4 does  600 ml of a 1.2...

How many g of H2SO4 does  600 ml of a 1.2 M solution of H2SO4 contain? Please show all steps.

Name, what''s you''re name

what''s you''re name

How is the mesoderm of triploblastic animals formed, Q. How is the mesoderm...

Q. How is the mesoderm (third germ layer) of triploblastic animals formed? The mesoderm shows from differentiation of endodermal cells that cover the dorsal region of the arche

Differences between budding and fission, Differences between Budding and Fi...

Differences between Budding and Fission Both budding and fission are identical in at least one way in that the young ones produced by these procedures are the result of direct

What is the ph inside the stomach, What is the pH inside the stomach? Why i...

What is the pH inside the stomach? Why is there a need to keep that pH level? How is it maintained? Which are the cells that produce that pH? The normal pH of the gastric juice

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd