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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Describe Assist Control Ventilation (ACV) The machine delivers a set tidal volume at the set rate; besides, each time the patient breathes on his own, a machine breath is init
F a t cow syndrome Fat cow syndrome, also known as fatty infiltration of liver, is a highly fatal disease in high yielding dairy animals that occurs a few days before or afte
Q. Common causes related with angina pectoris? • The usual cause of angina is the narrowing of the major coronary artery due to atherosclerosis. • Systemic hypertension inc
What is the Procedure of Normal saline dressing 1. First collect the supplies near the patient. Place should be clean with good lighting where it is easy to work. Use a newspap
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Two chromosomes in a cell have identical length and centromere position yet are not homologous, what is different about them.
Foscarnet (Foscavir) Foscarnet is an alternative to ganciclovir and valganciclovir for treatment of CMV infection. It is approved for use in CMV retinitis, including progress
Fluoride Inhibitor Fluoride inhibits enolase enzyme involved in the conversion of 2-phosphoglerate to phosphoenol pyruvate.
Explain Lamda and Kappa-carrageenan Lamda-carrageenan at a concentration of 0.05% produces thickening and stabilization. Sodium salts of Lamda and Kappa-carrageenan at a co
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