Autoradiography, Biology

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.

Posted Date: 12/10/2012 1:11:12 AM | Location : United States







Related Discussions:- Autoradiography, Assignment Help, Ask Question on Autoradiography, Get Answer, Expert's Help, Autoradiography Discussions

Write discussion on Autoradiography
Your posts are moderated
Related Questions
Menerals are naturally occurring inorganic solids with a crystalline structure and a definite range of chemical formula. Ores are concentrations of minerals in rock.

State the Classification of nervous system Neurological disorders can be categorised according to the primary location affected, the primary type of dysfunction involved, or th


Yeast is cultured in the presence of radioactive phosphate and the following biological molecules are purified. Which molecule should be most radioactive? A. An oligosaccharide B.

Q. What are the morphological, chemical and functional similarities and differences between peroxisomes and lysosomes? Similarities: Peroxisomes and lysosomes are small membran

Define Technique of Paper chromatography? Paper chromatography is used as 1. Ascending, 2. Descending or 3. Horizontal technique (circular paper chromatography) It is De

what is the excreatory organ of silverfish?

Blood Plasma - Circulation Centrifugation of blood results in its separation into two portions - a packed mass of cells which constitute around 45 per cent of the volume of t

Two major objectives must be achieved to treat IE effectively. The infecting  micro-organism in the vegetation must be eradicated. Also, invasive, destructive intracardiac and foca

What is the incubation period of an infection? Incubation period is the time interval among the infection by an agent that causes disease and the first signs or symptoms of th