Sequence a second set of fragments

Assignment Help Chemistry
Reference no: EM131128762

The Sanger dideoxy method for determining DNA sequence is limited in that a stretch of only 1000 or fewer bases can be analyzed in one reaction. Suppose you wish to sequence a newly isolated double-strand DNA tumor virus that contains 5000 base pairs. You decide to use the Sanger method on restriction fragments of the DNA for sequencing. You use an enzyme that makes a significant number of cuts to give, on average, fragments of 300 nucleotides or less.

Why might it be a good idea also to sequence a second set of fragments cleaved by another restriction enzyme?

Reference no: EM131128762

Questions Cloud

What is the free energy cost of pumping calcium ions : What is the free energy cost of pumping calcium ions out of a cell when the cytoplasmic concentration is 0.4 uM, the extracellular concentration is 1.5 mM, and the membrane potential is -60 mV?
Time management for effective results in your studies : Write an persuasive essay on the topic  "Time management for effective results in your studies"
What is a contingent liability : What is a contingent liability? Give an example of a contingent liability that is usually recorded in the accounts.
What entries should be made : Baylor University sold 10,000 season football tickets at $80 each for its five-game home schedule. What entries should be made .
Sequence a second set of fragments : Why might it be a good idea also to sequence a second set of fragments cleaved by another restriction enzyme?
Give the entry to record the proceeds : Planet Hollywood has cash proceeds from sales of $7,400.This amount includes $400 of sales taxes. Give the entry to record the proceeds.
Method to determine the sequence of adjacent genomic regions : If you have access to the genomic library of an unknown eukaryote and you know the sequence from a segment of its genomic DNA, devise a method to determine the sequences of the adjacent genomic regions.
Explain the principles relating to contract law : Explain the principles relating to contract law and the presentation slides must be in the IRAC method
Changes in usa education system : Write an argumentative essay on the topic "Changes in USA's Education System"

Reviews

Write a Review

Chemistry Questions & Answers

  Explain which bronsted-lowry acid is not considered

Which bronsted-lowry acid is not considered to be a strong acid in water. A)H I B)H Br C)H2S O3 D)H NO3

  State nitrogen oxide is a pollutant in the lower atmosphere

Nitrogen Oxide is a pollutant in the lower atmosphere that irritates the eyes and lungs and leads to the formation of acid rain. Nitrogen oxide forms naturally in the atmosphere according to the following endothermic reaction

  Define the energy associated with the transition

An electron makes a transition from n = 8 to n = 3. What is the energy associated with this transition

  Explain sequence for the conversion of benzoic acid

Propose a reaction sequence for the conversion of benzoic acid into phenylacetic acid. No need for mechanisms

  Define the molecular formula of the compound

A compound that is 43.64% P and 56.36% O by weight has a molecular weight of 283.88 g/mol. What is the molecular formula of this compound

  Explain what is the enthalpy of combustion of fuel

What is the enthalpy of combustion of this fuel in kJ/mol? The specific heat capacity of water is 4.184 J/g-°C.

  Explain steps take to increase the yield of the product

If the reaction is begun by adding the catalyst to a flask containing C2H6 at 10.0 bar, what will the partial pressure of C2H4 at equilibrium. Identify three steps the manufacturer can take to increase the yield of the product

  State activity coefficients in solubility-product expression

Find the concentration of Pb2 in 0.0010 M KI saturated with PbI2. Include activity coefficients in your solubility-product expression.

  Find the molecular and ionic and net ionic equations

Problem- Find the molecular, ionic, and net ionic equations for the following. Underline all solids. If no reaction occurs, complete the molecular and ionic equations, but write "no reaction" in place of the net ionic equation.

  Which intermolecular force is found in oil

Which intermolecular force is found in oil? Please explain your answe

  Explain what is the value of the equilibrium constant

For the cell reaction: Cu(s) + 2Fe3+ ? 2Fe2+ + Cu2+ Ecell° = 0.33 V What is the value of the equilibrium constant at 25°C for the cell reaction

  Te following experiment was carried out with a newly made

the following experiment was carried out with a newly made gaseouschlorofluoro carbon. 50.0 ml of the gas effused

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd