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Question: Assume you have isolated a cell population from bone marrow using as newly discovered CD antigen. You believe that this population has hematopoietic potential. When you grow these cells in culture, they have limited proliferation capacity, which you think qualifies them to be an adult type stem cell. Also, in vitro when differentiated, these cells can give rise to enucleated mature erythrocytes. You know that the ultimate proof to classify them as HSC is to show that they home to the bone marrow in vivo. Which experiment do you perform to show that these cells home?
Determine where are the hydrophobic or hydrophilic residues concentrated? Where does your mutation fall relative to these clusters and to the inside or outside of the protein?
Suppose that lions running on grass neither benefit nor are harmed by this interaction, but their activity helps the grass grow.
We require to make 200 mL of a 0.1 aqueous glycerol solution. In lab, we only have a dry glycerol powder, so you will require to make a 1.0M stock solution before you make the dilution.
If a transferrin receptor has a cross-sectional area of 50 Angstroms X 100 angstroms (5x10nm), and how many receptors could be accomodated in a 100nm clathrin-coated vesicle assume that only 50% of surface area can be occupied by proteins.
Was there any noticeable effect on the germination rate of the radish seeds as a result of the pH? Compare and contrast the growth rate for control with the alkaline and acidic solutions.
Specify whether the taste of fresh water surprised you or not? Explain if your perception of coarseness change or not?
In the year following the fires, wildflowers sprouted in all burned areas, closely followed in subsequent years by seedlings of the trees that normally grow in the area, such as aspens and lodge poles pines. What changes in the Ecosystem will take pl..
Ethanol is supplied in a 95 percent solution. You need 50mL of 70 percent Ethanol for your experiment. How would you make this solution?
A clinical trial is organized to compare an experimental medication designed to lower blood pressure to a placebo. Before starting the trial, a pilot study is conducted involving ten participants.
What is the difference between lactococcus lactis and staphylococcus epidermis? I am doing a lab and I've found out that my bacteria are gram positive cocci that ferment lactose and mannitol. I have it narrowed down to lactococcs lactis and staphyloc..
Compute the number of RBC if the dilution is 1:50, the cell count is 400 cells counted in five (5) RBC squares of the hemocytometer.
Explain why does depletion of Ca2+ from the medium through chelating agents promote isolation of cells from epithelial tissues during primary cell culture?
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