Purifying a protein on a weak cation exchanger

Reference no: EM1390077

Question: Assume you are purifying a protein on a weak cation exchanger (pH 7.5) and it comes off at 300mM salt. predict what salt concentraiton (lower, same, higher) the protein would come off if you used a strong cation exchanger. Explain the logic behind your prediction in terms of the differences between a weak and strong exchanger.

Reference no: EM1390077

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