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You're trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you're amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.
5. You have sequenced a human protein gene from genomic DNA. You would like to use E. coli as mechanism to express this protein. Identify and briefly describe two modifications you might have to make to make to your sequence in order to have successful expression.
6. Restriction fragment length polymorphism (RFLP) analysis is commonly used for tracing the relatedness of pathogenic microorganisms in food-related disease outbreaks. Often, this technology can allow epidemiologists to identify the source of the contamination and thus what foods may need to be recalled. Describe the molecular principles underlying RFLP and how they enable this type of epidemiological analysis.
When does crossing overoccur? The closer two genes are togetheron a chromsome, In F2s resulting from atestcross, what percent of recombinant phenotypes is expected ifthe genes under study are independtly assorting?
Suppose you have a set of four Hfr strains and you carry out a series of crosses with a multiply marked recipient that is auxotrophic for many different nutritional markers.
What were the key innovations that allowed adaptive radiation of modern plants? Why are bryophytes considered to be primitive plants? Why do bryophytes and ferns need water for reproduction?
What is a homin and how is it different from a hominine? and state mnemonic device to remember the difference between a homin and hominine.
If you look at photograph for a short time and then look awayfrom it, your trace memory of the image will
Which of the following types of mammals occur worldwide?
Intervening sequences(introns) in dna they have no corresponding segment in mature mRNA are
How can a voltage (a gradient of charge) exist, across a cellmembrane, when there are equal concentrations of cations and anionson each side of a cell membrane and across the membrane?
Which of the following body systems secretes hormones that help regulate how much we eat how quickly food and nutrients travel through the digestive system
Explain how the light reactions and Calvin cycle of photosynthesis are linked chemically, structurally, andfunctionally. Include in your answer the main reactants andproducts of each of the two phases of photosynthesis, and whereeach phase take pl..
A woman from the family with normal cholesterol marries a man with no LDL receptors, and all their children have cholesterol levels twice the normal of the mother.
Which of the following is descriptive of an R plasmid?
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