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1) In order to separate the RNA you majorly utilize a protein denaturant solution of the guanidinum salts. These chaotropic solutions denature by disrupting the H- bonding. The solutions also consists of a reducing agent i order to denature disulfide bonds. The final composition of the solution is : 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0 , 0.5%(w/v)SDS, 100mM β-mercaptoethanol. You have the guanidine isothiocyanate powder(mol.Wt.118.16),1 M Na Citrate, pH 7.0, powder SDS and b-mercaptoethanol(density 1.12 g/m L, mol. Wt.78.1). State how would you make up the 100 mL of this solution?
How did Thomas Hunt Morgan"s experiments with eye colour in Drosophila show the existence of sexlinked genes.
Describe the reason behind the difference in the physical properties of methane and methanol. Consider the lifestyle in a monastery, how does chant reflect life in the middle Ages.
What are advantages and disadvantages or observing living-specimens slides and heat-fixed-specimen slides of microorganisms. Which statements correctly describe the population of wolves.
Noah is a technician assigned to photograph chromosomes. At what stage of the cell cycle are the chromosomes sufficiently condensed that they FIRST become visible under light microscope.
How could you demonstrate experimentally that the selective antagonist binds the receptor specifically? Illustrate the experiment you would perform and also the controls you would contain.
Why can there be dissimilarity between heart rate averaged over 15 seconds and instantaneous heart rates. Suppose that green turtle grass ingestion time for the sea urchins has an approximate normal distribution.
A clinical microbiologist makes serial dilutions of several antimicrobials in broth, and then incubates each drug dilution series with a standard amount of a patient's isolated pathogen. What is this micro biologist setting up.
Is habitat temperature reflected in the genomes? What is presumably connected to thermostability.
What is the phenotypic ratio for the F2 generation? Demonstrate the work, including your key. what are Harry and Sally's blood types? Show your work. What if blood typing exposed Harry had type O blood.
After reading these articles, do you believe that it is the industry's fault or should the burden of responsibility be on the consumer who is making the food choices? You should provide a specific opinion to earn full credit.
What techniques would you utilize to detect this DNA sequence. Mechanical stress or trauma frequently triggers the symptoms of gouty arthritis in susceptible people. In this view elucidate why gouty arthritis affects the great toe more commonly than ..
A male rabbit with genotype GgBb. Find out the gametes produced by this rabbit (the sperm would have these combinations of alleles) Hint there are 4 combinations.
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