Identifying hydrolytic enzymes

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Suppose you poured iodine on your plate and notesed clearing in the uninoculated area, as well as around both of your transferred cultures. What are some possible explanations for this occurrence? Was integrity of the exercise compromised? What kind of things might be done to avoid this problem in future exercises?

How would you expect the results of this exercise to change if you were to add glucose to the medium?

In many tests it is acceptable to read a positive result before the incubation time is completed. Why is this not the case with Starch Agar?

Suppose you could selectively prevent production of a-amylase or oligo-1,6-glucosidase in an organism that normally hydrolyzes starch. Which enzyme would the organism miss the most?


Reference no: EM1396199

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