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In a cell biology laboratory experiment, two students independentlyisolated the enzyme lactate dehydrogenase, which catalyzes thereduction of pyruvate to lactate, from chicken heart. The enzymewas obtained in the form of a concentrated solution. The studentsthen measured the activity of their own enzyme solution as afunction of substrate concentration under identical conditions andfrom the data determined the Vmax and KM of their own preparation.When they compared results, they noticed that their values of KMwere identical, but their values of Vmax were radically different.One student argued that the different Vmax values confirmed thatthey had isolated different forms of the same enzyme. The otherstudent argued that, in spite of different Vmax values, they hadisolated the same form of the enzyme.
(a) Which student was correct? Why? (be explicit - useequations)
(b) How might they resolve the discrepancy?
Each of the following statements Explain a type of column chromatography . Sort the statements to the type of chromatography they describe. If statement can explain all of the types, place that statement in the category.
Explain the function of aminoacyl-tRNA-synthetase and why it is important that this enzyme is perfectly accurate in its recognition of tRNAs and amino acids.
Cells use transporters to move nearly all metabolites across membranes. But how much faster is a transporter than simple diffusion? There is sufficient information available for glucose transporters to make comparison.
what happen if any did the UMP have on the activity. An investigation was set up to observe the effect of varying concentrations of the substrate galactose(S) on the rate of reaction of the kinase,v, with and without an added artificial nucleoside ..
you wish to insert a human gene into bacterial DNA.Why would you use same enzyme to cut the human DNA as you selected to cut the bacteria DNA above? please only those who know the details.
They have one child. What is the possibility that their child will have the disease. 52 dark moths and 21 light moths were at random recaptured. Which would be the most reasonable assumption.
Erythropoetin is available as a pharmacological drug treatment. Recently a well-known cyclist was accused of using erythropoietin.
express "transamination" and "oxidative deamination"
Describe how protein hormones versus steroid hormones play a role in transducting signals that can affect gene transcription?
Use a chi-square test to evaluate whether the two loci are linked or assort independently.
What is the main purpose of the light-dependent reactions of photosynthesis?
In regards to various environmental epidemiologic topics, name three common challenges that relate to exposure assessment.
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