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1. How much 4X SDS-PAGE sample buffer do you need to add to 10 ul of a cell extract to run it out on an polyacrylamide gel?
2. You wish to plate out 5 x 104 lymphocytes in a total of 2 ml for a focus forming assay. You dilute your cells 1:100 with crystal violet and count them with a hemocytometer. The average cell count is 25. What volume of cells would you add to what volume of medium to prepare your assay? Show your work!
3. An antibody to be used for Western blotting needs to be diluted 1:5,000 for optimum results. What volume of the antibody stock solution do you need to add to 100 ml of buffer to make the blotting solution? By the way, what other components should this buffer solution contain?
4. You have 20 ml of a protein sample in a 10% (w/v) suspension of sodium sulfate. How many rounds of dialysis (in a total volume of 1 liter) would be needed to reduce the salt concentration to 1 mg/ml or less?
what are the chemical and functional similarities and differences between lysosomes and
What are endothelial cells? What role do they play in wound healing and in angiogenesis (define).
How may you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?
In rabbits, black hair is due to a dominant gene; brown is recessive allele. Short hair is due to a different dominant gene carried at a location o a different chromesome.
what are the chances of a baby to be a diabetic patient when one parent has diabetes the other parent doesn't?
A woman's father has ornithine transcarbamylase deficiency (OTD), an X-linked recessive disorder producing mental deterioration if not properly treated. The woman's mother is homozygous for the wild-type allele.
describe the concept of semi-conservative replication and the experiment that showed this. Describe the experiment that was used to demonstrate that DNA was replicated bi-directionally.
How can real-time quantitative PCR determine if one copy of a gene is missing? a. Only one quarter the amount of protein accumulates in the cell
q1. in 2009 a german politician proposed that all babies must be genetically fingerprinted at birth. what are the
Within the given DNA sequence, which strand of nucleotides would produce the amino acid chain shown below? (which strand functioned as the template?
What is the probability that his son would develop
The flagellated protest Giardia does not have true mitochondria but does have organelles called mitosomes. Determine what features would you look for to test the hypothesis that mitososmes are derived from mitochondria?
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