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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentration of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
Suppose that you have just discovered a new pathogen whose genome contains 29.1 percent A, 22.3% T, 25.8% C, and 22.8% G based on the relative molar concentrations of each nitrogenous base.
In an Oct. , interview with Professor Stuart Levy, a microbiologist at Tufts University, Ira Flatow, the host of the nrp program Science Friday, asked why physicians who treated victims of anthrax infection (or those who had merely been exposed) p..
What is the difference between impression fossils, index fossils, amber fossils and trace fossils.
A number of major worldwide industries are based on anaerobic respiration in a lower form of organism.define how anaerobic respiration plays a part.
A zookeeper hypothesizes that converting the intensity of the light in the primate exhibits will reduce the amount of aggression between the baboons.
A double stranded DNA virus has 200,000 base pairs.find out the number of nucleotides present.
Create a diagram for a hydrocarbon with 5 carbons with a double bond between carbons 2 and 3 and structural isomer with the same number of carbons and hydrogens.
The sequence below represents the middle portion of a gene (so don't look for primer or start sites). A frameshift mutation between base +10 and +11 adds a guanine between the two "c"s in the minus strand of DNA.What functional change in the prote..
What is the function of ribosomes in the rough ER.
If we wanted to produce human insulin or human blood clotting factor in bacteria, what problems might we encounter? (genetic problems, not ethical)
What is the best way to administer chemotherapy? Single agent or combination chemotherapy? Explain how signature sequences are used in bacterial identifications.
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