Determine which ion exchanger and optimum ph of the buffer

Reference no: EM1395292

A protein of interest has an exceptionally acidic pI of around pH 5.5 whereas the rest of the other proteins have an overall basic pI of around 7.5. Which ion-exchanger (cation or anion) and optimum pH of the buffer would you recommend so that the protein of interest would elute from the column without binding?

Reference no: EM1395292

Questions Cloud

Physics: simple harmonic oscillator : Physics: Simple Harmonic Oscillator, Traveling waves are set up in a pair of long strings by a simple harmonic oscillator. The strings have identical mass densities of 5.1 grams/meter.

Recessive mutations in the x-linked white gene : Wild-type fruit flies, D. melanogaster, have dark red eyes. Recessive mutations in the X-linked white gene result in white eyes.

Test value and the degrees of freedom : Given the variances of the two samples below, find the test value and the degrees of freedom that should be used in an F test.

Evaluating correlation articles : Search the library for a scholarly journal article of a study that presents the results using correlation analysis. Summarize the article, explain how the correlation was used, and the value of correlation analysis.

Determine which ion exchanger and optimum ph of the buffer : A protein of interest has an exceptionally acidic pI of around pH 5.5 whereas the rest of the other proteins have an overall basic pI of around 7.5.

Relationship among the units of heights of people : Using a spreadsheet, create a table that shows the relationship among the units of heights of people in centimeters, inches and feet in the range of 150cm to 2m. Use increments of 5cm.

Correlation coefficient for the data : Find the correlation coefficient for the data. Find the equation for the regression line for the data and predict the final grade of a student who misses 3.5 days.

Assessing the p-value for hypothesis test : using a significance level of .05, test the hypothesis that the intake of linoleic acid in this group is lower than that of the general population. Also, compute a p-value for the hypothesis test.

Why alcohol effective against some viruses and not others : Occasionally, subjecting a plant to photoflood and a fan will produce a lower rate of transpiration than exposing it solely to a photoflood. Why is alcohol effective against some viruses and not others?

User Account

All Pages