Determine the dissociation constant

Assignment Help Biology
Reference no: EM1385847

Question: A protein (R) is a trimer, which you suspect may exhibit cooperative binding. Each monomer contains a single binding site for the ligand, A. To investigate this you obtain the this data shown with [R] total=20uM. Make a hill plot of the data and determine the dissociation constant, Kd and the hill constant, nH. If present what type of cooperativity does R display with respect to A.

[A] total(mM) [A] bound (uM)

0.3 0.814

0.6 4.56

0.9 11.3

1.2 19.7

1.5 27.9

2 38.8

3 50.4

4 55.1

6 58.2

8 59.1

12 59.7

 

Reference no: EM1385847

Blood supply to the hearts myocardium

Blood supply to the heart's myocardium is directly provided by the _____ artery(ies).  Specialized cells of the right atrium that act as the heart's pacemaker are called the

Any interestingbiological or personal facts

What is Mendel most famous for? Any interestingbiological or personal facts? Where is he from, how did he get started in science, how has his contribution changed the world?

How many genes does this bacterial genome encode

Mycoplasmas are the simplest self-replicating (free-living) bacterium known. The Mycoplasma genitalium genome is 580,000 bases in length. Approximately how many genes does t

Transcription is the production of rna from dna

Transcription is the production of RNA from DNA. It reads one strand of DNA, called the template, to create a new RNA strand. The nucleotides are added to the growing RNA stra

Describe 2 types of natural selection

After a long scuba diving session on a Caribbean reef, David boards a plane to Denver. He begins to feel pain in his elbows on a flight back home to Denver.

Maximal voluntary contraction

What would happen to your subject's blood pressure if he or she were to perform a sustained hand grip with 25% of their maximal voluntary contraction(MVC). What if they were t

Determining the evolutionary standpoint

1. "If humans are in the same class as domestic cats we must also be in the same order." True or False? Explain. 2. Explain in your own words how, from an evolutionary standpo

Costs of territory defense and maintenance

What type of information is important for a territorial animal to learn before territorial establishment rather than after - difference between piloting, path integration and

Reviews

Write a Review

 
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd