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Question: A protein (R) is a trimer, which you suspect may exhibit cooperative binding. Each monomer contains a single binding site for the ligand, A. To investigate this you obtain the this data shown with [R] total=20uM. Make a hill plot of the data and determine the dissociation constant, Kd and the hill constant, nH. If present what type of cooperativity does R display with respect to A.
[A] total(mM) [A] bound (uM)
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