Determine the concentration of your overnight culture

Assignment Help Biology
Reference no: EM13118983

1) Assume that you measure the OD of an overnight E.coli culture. The spectrophotometer gives you a reading of 2.8. What would you do to determine the concentration of your overnight culture and why?

2) The OD of an E.coli culture is 0.23. You transfer 300 µl of this culture into 29.7ml of medium. You continue this same dilution one more time. You transfer 50 µl of your last dilution onto an LA plate. After 24 hours of incubation, you observe 11 colonies on this last plate. A) Did you expect this result? If not, name at least three reasons for this result.

3) A friend of yours asks you to help him/her draw a bacterial growth curve. He/she gives you the following information: the TA started a fresh culture at 8:30am (the OD of this fresh culture was 0.14). During class the following OD readings were obtained: 0.19 at 9:50am, 0.28 at 10:10am, 0.29 at 10:30, 0.68 at 10:50 am, and 1.1 at 11:30am. At 11:30 am the OD of a 1:10 dilution of the stock culture was 0.29. A) Draw the graph. B) What was the generation time of this culture?

4) For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD is 0.89. A) In order to calculate the concentration of the Saccharomyces cerivisiae culture, can you use the equation that you use to calculate the concentration of an E.coli culture? If yes or no, give a detailed explanation. B) Does E.coli or Saccharomyces cerivisiae have a shorter generation time?

Reference no: EM13118983

Why are the genes involved in lactose metabolism

Why are the genes involved in lactose metabolism considered to be an operon? Choose one answer. a. They are under the control of the same promoter. b. They occupy adjacent

Phenomenon of bacterial resistance to antibiotics

Think about the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this condition?

Draw a pedigree for this family

The M and N blood type alleles are codominant. At the unlinked 1 locus determing the A/B/O blood type, alleles IA and IB are codominant, but both are dominant to i. Both Par

Microevolutionary changecausing macroevolutionary speciation

Describe the evolutionary development of either the horse orelephant from its pre-historic to present-day form, using 2specific features that have exhibited microevolutionary

Explain how the poly binding protein

Explain how the poly(A) binding protein that binds to the poly(A) tails located in the 3' end of an mRNA can play a key role in an mRNA degradation pathway that proceeds from

Design and execution of digestion trials

Extimate which well-known goat feeding strategy combined with which errors in the design and execution of digestion trials led some animal nutritionists to believe that goats

Please separate the report totally 5

Discuss on your observation of direct and indirect staining samples 2 sample, I with crystal violet and congo red. using 40x magnification and 100x magnification.  And Differe

Movement in the last decade encouraging

One: There has been a movement in the last decade encouraging people to "buy American." Discuss the legal and ethical issues related to boycotting goods from other countries.

Reviews

Write a Review

 
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd