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Question: Assume you have 2 tubes containing cultures of genetically distinct bacterial strains. You know that when you mix the two together, you get Horizontal gene transfer (HGT), but you do not know what genetic exchange mechanism is accomplishing this transfer. Prior to mixing the strains, you add an enzyme that degrades any DNA it comes into contact with the tubes and allow it to incubate briefly. You then mix contents of both tubes together and find that you are no longer able to achieve HGT. Both cultures are still completely viable. Name the 3 methods of HGT that we discussed in class. Which of these is the MOST likely process you interfered with? In your answer, explain your observations and why the other two processes are not likely to be involved.
A 5.0 mL aliquot of a stock tryptophan solution was diluted by adding 95 mL of water. The absorbance of that diluted solution was measured at 280 nm as 0.055 absorbance units.
Explain how the cell anatomy of the non-ciliated simple columnar epithelial lining of the small intestines cpmtributes to the overall function of the small intestines.
Would the blood pressure in pulmonary capillaries at the bottom of a lung be the same as blood pressure in the capillaries at the top of a lung in the standing individual?
Compute the frequencies of the genotypes in future generations if the initial population consists of the following genotype frequencies.
Explain this dosage strategy in terms of the negative feedback control of cortisol secretion and receptor function in hormonal pathways.
Large folds in the stomach known ______ increase its surface area and also enable it to fold on itself when empty. The ______ is a valve that enables partially digested food out of stomach into the ______.
explain the advantage and disadvantage of random addition or deletion of nucleotides at VDJ junctions during re-arrangement of immunoglobulin gene segments.
If a transferrin receptor has a cross-sectional area of 50 Angstroms X 100 angstroms (5x10nm), and how many receptors could be accomodated in a 100nm clathrin-coated vesicle assume that only 50% of surface area can be occupied by proteins.
Compare changes in carbon cycle to that of the nitrogen cycle on human health. Create a conceptual graphical model for how changing the carbon cycle is likely to impact human health.
Primary transcripts produced by RNA polymerases are generally faithful copies of the DNA template. However, most final/functional forms of these RNAs vary substantially from the primary transcript.Give an instance of an RNA class.
Discuss three adaptations for terrestrial life that occurred during the evolution of green plants. For each adaptation, note the taxonomic group in which the trait first appeared
Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
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