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bioinformatic You have been given a gene sequence, which is in RAW file format. Perform the following tasks by visually inspecting the sequence using information you were provided during lectures for Module 1, Topics 2 and 3. CCCTGGTTATTTATTGACAGGGGTATATAGTCCAGTGGTAGGCTGGATTA TATAATTTATTGAAATTTTCGATAAATTCCGTTATAATAATATTAAATCT GAAAATATTTAGTAAAAGGATGTGAGATATGTGAAGCTAAAACGCTTGTC TTTCTTCATGTTTGTTACCTTACTGGTATTTATATCTGTTTTTCCTGTTT ATGCAAACGATTTCGAAAAACATGGTACATATTATGAAATTTTTGTCAGG TCTTTTTATGACTCTGATGGTGACGGAATAGGGGATTTGAAAGGTATAAT AGAAAAACTGGATTATCTTAATGATGGAGACCCTGAAACCATTGCTGATC TGGGGGTTAATGGTATCTGGTTAATGCCTATCTTTAAATCTCCCTCCTAT CATGGCTATGATGTAACCGATTATTATAAGATTAATCCTGACTACGGGAC TCTGGAAGACTTCCATAAGCTTGTTGAGGCTGCCCATCAAAGGGGAATCA AGGTTATAATTGATTTACCCATCAATCATACCAGTGAAAGACATCCCTGG TTTCTCAAGGCTTCCCGGGATAAGAATAGTGAATACAGGGATTATTATGT CTGGGCTGGCCCCGATACCGATACCAAAGAAACCAAGTTAGATGGAGGCC GGGTCTGGCATTATTCCCCGACCGGCATGTATTATGGGTATTTCTGGAGT GGCATGCCTGATTTAAACTATAATAACCCTGAAGTTCAGGAAAAGGTTAT TGGGATAGCAAAATACTGGTTAAAACAGGGGGTTGATGGTTTCAGGCTTG ATGGAGCCATGCATATCTTCCCACCGGCCCAGTATGATAAAAACTTTACC TGGTGGGAGAAGTTCCGTCAGGAAATTGAAGAGGTAAAACCCGTTTACCT GGTGGGTGAGGTCTGGGATATTTCGGAAACGGTAGCTCCTTACTTCAAAT ATGGTTTTGATTCTACCTTTAACTTTAAACTGGCAGAGGCAGTTATCGCT ACGGCTAAAGCTGGATTTCCCTTTGGTTTTAATAAAAAGGCAAAACATAT TTACGGGGTATATGACAGGGAGGTTGGATTTGGGAATTATATCGATGCTC CCTTCCTGACCAACCATGATCAGAACCGGATTTTGGACCAGCTTGGGCAG GATCGTAATAAGGCCAGGGTTGCTGCCAGTATTTATTTGACCTTGCCTGG TAATCCCTTTATTTACTATGGTGAAGAAATCGGTATGAGGGGGCAGGGGC CCCATGAAGTTATCAGGGAGCCCTTCCAGTGGTATAATGGATCCGGGGAG GGAGAAACATACTGGGAGCCAGCCATGTATAATGATGGCTTTACTTCTGT TGAACAGGAAGAAAAGAATCTCGATTCCCTCTTAAATCACTACAGGAGGT TAATCCATTTCCGGAACGAAAATCCTGTCTTTTATACCGGTAAGATTGAG ATTATAAATGGAGGATTAAATGTAGTTGCATTTAGAAGATATAATGATAA GAGGGATTTATATGTCTACCATAACCTGGTAAACAGACCGGTTAAAATAA AAGTGGCTAGTGGTAACTGGACCTTATTGTTTAATTCAGGTGATAAGGAA ATTACCCCTGTTGAAGATAATAATAAACTTATGTATACTATCCCTGCTTA TACTACCATTGTTCTGGAAAAGGAGTAAAGGGAGAGGGTGAGTAATAATT ATGAAAAGGAGAGGGTAAATCCCTCTCCTTTTTTGTTAAA (a) Identify the possible Open Reading Frame (ORF). Hint: search for the START and STOP codons. (b) How many amino acids will the gene code for? (c) Translate the sequence and deduce the amino acid sequence for the putative protein. (d) Can you identify a signal peptide? If yes, identify it? (e) Sometimes, it is not possible to identify a gene using the method you used in question (a) above. One of the reasons is that the gene has not been sequenced completely - this happens during random sequencing of clones from bacterial genomic libraries. Perform a 6 frame translation of the given gene. Can you see the difference between the amino acid composition of all 6 translations. (f) Determine the G+C content of the coding region of the gene. Change the G+C content of the gene without changing the amino acid composition.
How could you demonstrate experimentally that the selective antagonist binds the receptor specifically? Illustrate the experiment you would perform and also the controls you would contain.
Why is the immune system so highly conserved, as other physiological systems are more specially adapted to our mammalian ancestry or hominid roots.
What percent of all the enzyme molecules will have their Cys thiol in the active form at pH 8.6. Some mammals might run or fight when frightened. For both of these examples, illustrate the physiological mechanisms involved in the answer.
Genetic diversity is necessary for natural selection to act, but natural selection can decrease or eliminate diversity. What procedure can restore genetic diversity to a population.
Who was Carl Weigert? What influence did Weigert have on Ehrlich. Other than the acid-fast stain, what other technique may be used to diagnose tuberculosis? What scientist developed this test.
This rise is proportional to the starting concentration of sucrose in the solution. A metabolic poison that blocks the ability of cells to regenerate ATP is found to inhibit the pH changes in the solution. Recommend a hypothesis accounting for these ..
The brown and scarlet mutations assort independently. If fruit flies that are heterozygous for both of these mutations are intercrossed, what sorts of progeny will they create, and in what proportions? Describe how you got the answer.
How will a scientist predict the relative frequencies
The energies of glycolysis shows that there is a large drop in free energy upon oxidation of glycerladehydes 3-phosphate to 1,3-bisphosphoglycerate. In the presence of oxygen, some of this energy is ultimately converted into ATP production. though..
A) What is the broad sense heritability of seed count in this population of bluebonnets? B) What might cause this estimate of heritability to be inaccurate?
Calcium Polycarbophil is calcium salkt of polyacrylic acid cross linked with divinyl glycol. But what is its forumla.
These experiments demonstrated that DNA is the genetic material of phage and that protein does not transmit genetic information. Can this same experiment be carried out with 35S and 14C? Describe it.
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