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You are interested in performing a mutagenesis study. You design oligonucleotide primers and perform a polymerase chain reaction (PCR) to induce a mutation of interest into a synthetically generated PCR DNA product. The DNA template used in the PCR reaction is derived from E. coli cells and harbors the wild-type sequence of the mutant protein you are interested in studying. At the end of the PCR reaction, you recognize that in addition to the synthetic product harboring your desired mutation, you also have the wild type template DNA in your reaction test tube. However, you do not want the wild-type DNA to remain in your PCR reaction test tube for fear that it will contaminate your mutagenesis results. You try to think of ways to destroy the contaminant. You perform a Google search and discover the existence of an enzyme that acts to specifically degrade methylated DNA. Eureka! You think you have discovered a way to solve your problem of wild-type DNA contamination in the reaction. So you decide to use the DNA methylation degradation enzyme to add it to your test tube of PCR reaction product in efforts to destroy the original wild-type template DNA. You are confident that your mutated PCR product will be spared in this process.What are the differences between the wild-type DNA template and the synthetically mutated PCR product as it relates to DNA methylation (Which type is methylated? are both methylated? are neither methylated?). Provide a rationale for your response.Are you correct in assuming that using the DNA methylation enzyme will degrade the wild-type DNA only while sparing the synthetically generated PCR product? Justify your response.
Use a flow chart or numbered list, trace the path of a water molecule through a plant. Begin with a water molecule in the soil and end with the water molecule as water vapor in the air.
In the Cori cycle, when glucose is reduced by glycolysis to lactate in muscle, the lactate is excreted into the blood and returns to the liver where it is converted back into glucose by gluconeogenesis.
how to express the number of types of gametic chromosomal.
Compare and contrast gene expression in prokaryotes vs. eukaryotes from the standpoint of promoter sequences
Our heart pumping and our circulatory system keep us alive. Many things like poor diet, lack of exercise, drinking and drugs damage our systems, even to the point of early death.
The Neurospora cross was made between a strains that carried mating type A and mutant allele arg-1(arg) and another strain the carried mating-type a and the wild type arg-1 (+) gene. Four hundred linear octads were isolated and they fell into the 7 c..
Chooses the statement that best describes a buffer. A buffer prevents pH of a solution from changing while an acid or base is added.
What is the piston height h if the temperature is 30 degrees? How far does the piston move if temperature is increased by 200 degrees.
A recent report hypothesized that the IQ of children that are first-born differs from those that are born later. They found that first-born children have higher IQ’s than later born children, but you do not believe this is correct.
Which receptors were monitoring the drive, still the conscious part of your brain was asleep? Explain the proper answer.
A population of collared lizards is fragmented into 3-glade populations through an aggressive anti-forest-fire campaign. At the "B" locus, there are only two alleles
A man and a woman who each have wild type phenotypes have a son with Klinefelter syndrome who has hemophilia. Determine the genotypes of the parents?
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