What is the use of having restriction enzyme sites

Assignment Help Biology
Reference no: EM1395676

What is the use of having restriction enzyme sites at the 5' beginning of the luciferase gene and a set of restriction enzyme cut sites at the 3' end of the luciferase gene.

Reference no: EM1395676

Questions Cloud

Illustrate what you must do are intentionally vague : So many people are going to a small island which you must use two different hotels. You must use Microsoft Office, as described below, to keep track of all the details.
Which is normally killed in an hour when growing in a medium : Suppose you have a bacterium, which is normally killed in an hour when growing in a medium containing penicillin. This bacterium is an auxotroph and requires tryptophan for growth.
Explain the process of prokaryotic replication : Explain the process of prokaryotic replication. Pay particular attention to the early stages. In your work, be sure to include the origin of replication, DnaA, components.
Larger sample and standard sample surveys : That's a much larger sample than standard sample surveys. In spite of this, we can't trust the result to give good information about any clearly defined population. Why?
What is the use of having restriction enzyme sites : What is the use of having restriction enzyme sites at the 5' beginning of the luciferase gene and a set of restriction enzyme cut sites at the 3' end of the luciferase gene.
Illustrate what is the industry net after-tax interest cost : Assuming which Charter is in a 35% corporate tax bracket, illustrate what is the industry's net after-tax interest cost associated with this bond issue?
Which of the promoter elements are always present : Considering an E. coli promoter that could be recognized by a ?70-containing RNA polymerase holoenzyme, which of the promoter elements are always present, and which can be missing or altered? How does this E. coli promoter compare to a typical euk..
Advantages of master patient indexes : Do you believe the advantages of master patient indexes outweigh disadvantages, or have medical records gone too far in documenting and sharing private information?
What step in the rt mechanism controls the rate : What step in the RT mechanism controls the rate at which RT can proceed at incorporating the dNTPs.

Reviews

Write a Review

Biology Questions & Answers

  Purified protein concentration

You measured the purified protein concentration, which is 5 ug/uL. You decided to load 20 ug protein to the gel. How would you dilute your sample?

  What is the final concentration be in micromolar

what is the final concentration be in micromolar

  How prokaryotes generate genetic variation in population

Genetic variation is important in reproducing populations because it allows the population to adapt to changing environments. Those individuals who are genetically best adapted to an environment have a greater tendency to survive and reproduce.

  Discuss properties of the wild type amino acid

Discuss the properties of the wild type amino acid? Properties include charge, shape, size and flexibility, features unique to the amino acid.

  Primary treated sewage on biochemical oxygen demand

Discuss the effect of discharging primary-treated sewage on the biochemical oxygen demand (BOD) and dissolved O2 of the receiving body of water?

  Dihybrid two plants is done and seed pod shape is examined

A dihybrid cross of two plants is done and the seed pod shape is examined

  What will be a tentative diagnosis and suggested treatment

The both endocrine and the nerve system are major regulating system of the body; however, the nervous system has been compared to an airmail system and the endoncrine system to the pony express. What is the resemblance.

  Difference between eukaryotic and prokaroytic cells

Explain the differences between eukaryotic and prokaroytic cells. Include in your discussion three phenotypic systems used to classify microbes.

  What is the genetic basis of two pairs of alleles

A patient has an 80 percent blockage of his left anterior descending coronary artery. Illustrate what occurs in terms of myocardial oxygen supply and demand if his sympathetic nervous system is stimulated.

  Mutational event to the frameshift mutant

Some amino acids are post-translationally removed from the C-terminal end of the b-lactamase enzyme from B. imaginarium after translation and release from the ribosome.

  What abnormality may underlie those conditions

How can you infer what type of volcano erupted in a given area based on type of volcanic deposits now found as layers of rock. What abnormality may underlie those conditions.

  The most important strand synthesized continuously

Suppose the diameer of a neutral helium atom is 1.40 x 10 to the second power pm. supposed that we could line up helium atoms side by side in contact with one another. How numerous atoms would it take to make the distance from end to end 9.00cm.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd