What is the function of reverse transcriptase

Reference no: EM133322353

Question 1. How will you differentiate between COVID-positive and COVID-negative samples after we run the agarose gel next week?

Question 2. Why is it important to use a primer set that produces a band in the COVID-negative samples?

Question 3. What information do the positive (+) and negative (-) PCR controls provide?

Question 4. When performing most PCR experiments, and additional negative control is included that has a water template in place of DNA. Why would this be a useful control?

Question 5. What is the function of reverse transcriptase? Why did we have to use reverse transcriptase procedure with the samples we originally extracted from patients P1-P3?

Question 6. What chemicals and molecules are needed for PCR, and what is the function of each component?

Reference no: EM133322353

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