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Q. Eighteen museum specimens of the extinct great Auk were used to amplify coxI, a mitochondrial gene for cytochrome oxidase. DNA sequence analysis of 600 nucleotides of coxI revealed 5 alleles and the number of nucleotide differences between them are shown below, as well as the number of times each allele was found in the sample.
A B C D E NumberA 4B 4 3C 2 3 6D 2 4 1 3E 5 3 3 2 2
What is nucleotide diversity (π) of coxI in the Great Auk based on this sample.
Ralph is taking scuba diving classes, and is warned not to hold his breath as ascending from depth but to continuously release air from his mouth. What is the basis of this instruction and what is risk if it is ignored.
Calculate how the permeability of each is altered when the pH is reduced to 1.0, a value typical of the stomach.How to demolish the function of this protein.
The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar through pour plate method. The plates were incubated aerobically for 48 hours at 37C.
Madagascar, Indonesia and the Amazon basin have some of the greatest numbers of different species concentrated in a single area. What kinds of environments do you think encourage greater biodiversity.
Would you expect the measurements to be equivalent in both preparations? If a variation in size is expected, how will you account for this difference.
In a population with two alleles, A and a, frequency of A is 0.2. Organisms that is homozygous for A die before reaching sexual maturity. In five generations, what will be the frequency of individuals aa with genotypes.
The membrane vesicles are next washed, the membranes solubilized and the proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein.
How would you answer to a person who argued that there is nothing to worry about because natural systems, through natural succession, will heal the wounds of human activities and restore the balance of nature.
How denaturing a protein alters the functions of that protein. If we are bleeding by the accident, why the injury has to be deeper than epidermis.
Compute the change in energy of an atom that emits a photon of wavelength 2.21 meters. (Planck's constant is 6.626 x 10-34 joule seconds; the speed of light is 2.998 x 108 m/s)
Results of this analysis show that one of the most rapidly reassociating classes of DNA is substantially reduced in the older individual with respect to the 2 year-old. How can you describe this finding.
What must the intracellular 3-phosphoglycerate/1, 3-diphosphoglycerate ratio be to make the phosphoglycerate kinase reaction thermodynamically favourable in the direction of 1, 3-diphosphoglycerate synthesis.
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