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Stock of plasmid dna

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You are ready to set up your reaction.  You are provided with a stock of plasmid DNA that is at a concentration of 1mg/ml.  You have stock enzyme buffer that is 10X (meaning it is ten-times more concentrated than you need it to be in the actual reaction).  You have deionized water (dH2O) to use, as needed.

  • How many ml of DNA will you add to your reaction tube?
  • How many ml of 10X buffer will you add to your reaction tube?
  • How many ml of  ApeKI will you add to your reaction tube?
  • How many ml of dH2O will you add to your reaction tube?
  • At what temperature will you incubate the reaction?

So how would you solve for these?

Reference no: EM131437313

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