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If you had a solution of proteins from a crude extract in water, and added ammonium sulfate to 60% saturation, proteins with more hydrophobic surfaces exposed precipitate. If you then heated the solution to 60 degrees Celsius for 2 minutes, and then put the solution on ice, less-thermostable proteins will denature, and more more hydrophobic areas will be exposed on these proteins. Meanwhile, more-themostable proteins will not be denatured and remain in their native state. If you were designing a purification procedure to separate proteins on thermostability, after centrifuging, would you expect the less-theromostable proteins that are denatured to become less soluble and precipitate? Would this 60 degree Celsius heat treatment step cause more proteins to precipitate and become part of the pellet than before the step? How would you draw a flow chart for this step of a purification procedure?
Early one morning Officer Smith was sitting in her patrol car observing traffic near the town's elementary school when a sports car suddenly sped past. Realizing that students may be crossing the same street on their way to school, Officer Smith quic..
Can adjustments to environmental conditions (such as temperature changes) play an important role for mammalian circadian systems as a zeitgeber? Does this have anything to do with seasonal affective disorder in humans?
Yet there remains a great deal of genetic variation within populations. Describe some of the factors that contribute to this variation
In which organism will a transposon more easily spread: in an asexual haploid species, or in a sexual diploid species? Explain.
If a woman has blood type AB and her husband has blood type O, what is the percent chance that a child of theirs would have blood type A? (Hint: punnett square would be useful)
What specific term or structure is defined by each of the following?
Approximately how many people in sub-Saharan Africa are infected with HIV? How many died in the last year?
Explain why it is acceptable to record positive test before the suggested incubation time is completed but it is not acceptable to record a negative result early.
A persons normal tidal volume is 400 mL with a dead space of 100 mL. The respiratory rate is 12 breaths/min. The person is placed on ventilator for surgery and the tidal volume is 700 with a rate of 12. What is the approximate alveolar PCO2 for th..
Why does fermentation preserve foods and why would a farmer purchase Rhizobium? Bacillus thuringiensis - most of the world's population relies on wheat for food.
Suppose we use X-ray crystallography to determine the three dimensional structures of leucine and histidine at pH 8. This technique enables the determination of the positions of all the C, N, and O atoms in these two amino acids, but the hydrogen ..
the earths largest phylum is arthropoda including centipedes millipedes crustaceans and insects. the insects have shown
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