Shotgun approach to whole genome sequencing

Assignment Help Biology
Reference no: EM1397530

In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.
What are the steps in the shotgun approach to whole-genome sequencing?
PLACE IN ORDER
A. 1-kb fragments are cloned inot plasminds.
B. Chromosomes copies are broken into 1-kb fragments
C. The plasmids are sequenced.
D. Multiple copies of the smae chromosome are prepared.
E. A computer combines the fragments sequences.
F. 1-kb fragments are transcribed into RNA.

Reference no: EM1397530

Questions Cloud

Significant difference in the average prices : Is there a significant difference in the average prices for these two different supermarket chains? Test at α = 0.05
Determine the mole fraction biphenyl : Write down the balanced chemical equation when Fe 3 O 4 reacted with carbon monoxide and calculate mass of carbon dioxide that will be produced in this reaction if  234 g carbon monoxide is reacted with excess Fe 3 O 4 ?
Description of one way anova : Three different methods for assembling a product were proposed by an industrial engineer. To investigate the number of units assembled correctly with each method
Explain that how hcl could test for limestones presence : Explain briefly that how HCl could test for limestone's presence , use the balanced chemical equation for this and then describe specific reaction that takes place?
Shotgun approach to whole genome sequencing : In the shotgun approach to whole-genome sequencing, random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence.
Stimulation of the right vagus versus the left vagus : What is the difference between stimulation of the right vagus versus the left vagus? How are they connected to the heart? What would you expect to see upon left vagus stimulation?
Discuss increased importance for patient x to start diet : Patient X's doctor has been trying to convince him to practice better lifestyle habits to for several years to help combat his hypertension on antihypertensive medication for 3 years.
Appropriate hypotheses using appropriate data : Suppose that it is important to demonstrate that the wall thickness exceeds 4.0 mm. Test appropriate hypotheses using these data. Draw conclusions at α= 0.05.
Create determination of how much risk is acceptable : In assessing risk to benefit, on what bases do you make final determination of how much risk is acceptable and how much benefit is essential?

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd