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A student attempts to transform a population of E. coli with a pBluescript plasmid. How do you advise him to identify cells that have the plasmid? Answer Plate the cells on a medium containing X-gal. Cells with plasmids will turn blue. Plate the cells on a medium with ampicillin. Cells with plasmids will grow. Plate the cells on a medium with the restriction enzyme EcoRI. Cells with the plasmid will grow. Infect the cells with bacteriophage T3. Cells with the plasmid will transcribe the inserted gene and not be able to grow. Isolate individual colonies and prepare DNA from each. Size the DNA on an agarose gel to see if plasmids are present.
In "Chardakov method for water potential measurement" experiment, you find that 0.30 M solution has not been changed by water loss or absorption by the potato in it. Determine the osmotic potential in Mpa of this solution (0.25 M = -5.75 Bars).
Nucleus, Mitochondria, How do receptors interact with hormones? How do the hormones interact with each Other? How do the secondary messengers work Again? Hormone binds to G protein what is the Hypothalamus.
The genes dumpy wings, clot eyes, and apterous wings are linked on chromosome II of Drosophila. In a series of two-point mapping crosses, the genetic distances demonstrate below were determined.
What does it mean to be an "multicellular organism"? The cellular slime mold Dictyostelium discoideum exists initially as a collection of separate cells moving over the forest floor.
Think about a diploid organism with the genotype BbGgNnZZ. List all the different gamete types that this individual can produce.
suppose random and equal distribution of nucleotides.
Suppose you are interested in studying dogs as a system for obesity. You crossed a Cocker Spaniel to a Labrador Retriever and interbred the offspring for many generations.
why is the disk-diffusion technique not a perfect indicator of how the drug will perform in vivo? What other factors are considered before using the antimicrobial agent in vivo?
A store clerk tells you that vitamin C extracted from rose hips is better for you than synthetic vitamin C. Based on what you know of the structure of organic compounds, does this claim seem credible? Why or why not?
What impact has society had (or still have) on the progress of scientific inquiry? How do ethical or moral values affect research and the sharing of scientific information? How can this be resolved.
A nutrient agar plate labeled 10^-5mL had 154 colonies after incubation. what was the cell density in the original sample? what volume was used to inoculate this plate?
I just did a lab with antiseptics and disinfectants and a lab report question asks "were any microorganims increasing within the zone of inhibition of any of the disenfectants or antiseptics?
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