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You started work in a transcription lab working a with a novel bacteria phage. You isolated an enzyme that has RNA polymerase activity, and you discovered that your new viral RNA polymerase has a unique property. Unlike E. coli RNA polymerase, which prefers starting transcription with ATP, your new viral polymerase prefers starting transcription with GMP. You immediately go to your advisor and tell him that, based on what you learned, you know the directionality of RNA chain growth (3'-to-5' or 5-to-3') by your new RNAP.
1. Explain in what direction (3'-to-5' or 5-to-3') this enzyme synthesizes RNA and how you know this.
2. What other simple experiment can you do to show that your conclusion is correct, provided you have full access to all rNTPs and 2'-dNTPs?
All neurons are neural tissues, but not all neural tissues are neurons! Identify and discuss the roles of our different neural tissues, giving practical examples with respect to location and function as you go!
Describe the process of viral replication; discuss both normal and retroviruses, and comment on the implications of this process for genetic engineering.
Why is it essential that the reduced TTC be insoluble? Why is there less concern about the solubility of the oxidized form of TTC?
Retinal detachment from the choroid coat Why
Consider a population of 600 individuals, at Harfy Weinberg equilibrium. The two alleles in the population, B and b, cause two distinct phenotypes. Individuals who are BB or Bb are brown whereas individuals who are bb are yellow.
The view of anti-psychiatrists like Scheff was that mental illness was:
How is research important in preventing disease and/or the spread of disease? Give an example of how research has helped improve the outcomes of people with certain diseases. Also, explain how scientists determine if research sources and/or da..
RNA molecules can be both self-replicating and catalytic. This probably means that
A double stranded, relaxed circular DNA (2800 bps) is placed in a solution which favours the formation of B-DNA. What are the values for L, T, and W? Briefly explain your answer.
We have already been introduced to the heat of vaporization of water which equals 2404.2 kJ/liter, i.e. it takes 2404.2 kJ of heat to convert a liter of water into vapor.
Illustrate a 3C long carbon skeleton with the following functional groups attached. Include H atoms to fill the C atoms where necessary.
How many BamHI sites are there in this circular plasmid and where are those BamHI sites? Please draw the map.
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