Hydrolytic driving force, Hydrolytic driving force. The

Hydrolytic driving force

Reference no: EM1381941

1. Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA. THis hydrolytic reaction is catalyzed in E. coli by a pyrophosphatase that has a mass of 120 kd and consists of 6 identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10µmol of pyrophosphate in 15min at 37degrees celsius under std assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.

(a)How many moles of substrate are hydrolyzed persecond per milligram of enzyme when the substrate conc is much greater than KM?

(b)How many moles of active sites are there in 1mg of enzyme? Assume that each subunit has one active site.

(c)What's the turnover number of the enzyme? Compare this value with others mentioned in this chapter.

Reference no: EM1381941

Questions Cloud

Correlation between religion-social class : It has been said that Sunday is the most segregated day of the week. We know how segregated it is by race, but how is it segregated by class?

What percentage of the patients requiring trauma bays : When a patient is sent to another hospital, the hospital has to put a staff with adequate skills to guarantee the patient's life, which costs the hospital $200 per patient sent

Benefits of government-funded research : How does government funded research, help to enhance military power and enhance economic competitiveness?

Changing its formal employee relationship : Benjamin is a long-time key salesman for Morton and Dunderfield, a business supply company in Eastern Pennsylvania. The company decides to change its formal employer/employee relationship with all of its sales people, including Benjamin.

Hydrolytic driving force : Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA.

Write multiplication program make positive one-digit integer : Write down program which will aid the elementary school learn multiplication. Use Random object to make two positive one-digit integers.

Evaluate one or two of the three primary levels : Evaluate one or two of the three primary levels of union-management decision making. Include the following: can this level be improved, and if so, how

How common are coiled-coil motifs : Discuss how common are Coiled-coil motifs? Where are they found? Give two examples. Name four experimental techniques to determine protein protein interactions other than X-ray & NMR techniques.

Debate of immigration policy : Should the American government policy favor certain kinds of immigrants? Should preference be given to the neediest applicants? The most talented?

User Account

All Pages