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A generalized transducing phage first infects a bacterial donor of genotype his+, trp+, and rif+. After lysis of the bacterial donor, a bacterial recipient of genotype his-, trp-, and rif- is infected and incubated with the same transducing phage. The cultures from this second infection are split into three fractions and plated on three different media conditions to select for transduction of each marker individually. Then each of those plates is replica plated on media lacking histidine, lacking tryptophan or containing rifampicin. The colonies observed on these replica plates are used to infer the genotypes listed below
Fraction 1
Fraction 2
Fraction 3
his + trp+ rif+
19
trp+ his + rif+
10
rif+ trp+ his+
13
his + trp+ rif-
trp+ his + rif-
20
rif + trp+ his-
7
his + trp- rif+
33
trp+ his - rif+
5
rif + trp- his+
25
his + trp- rif-
35
trp+ his - rif-
65
rif + trp- his-
55
What media was used to select for transductions in the first plating of each of the experiments? [6 points]
Fraction 1:
Fraction 2:
Fraction 3:
What is the order of the bacterial genes? (circle)
It is the accumulation of acetyaldehyde that results in flushed face, dizziness, and light headedness responses that are associated with ethanol intoxication.
Assume you are interested in performing a mutagenesis study and you design oligonucleotide primers and perform a polymerase chain reaction to induce a mutation of interest into a synthetically generated PCR DNA product.
If a long-haired black male is crossed with a tortoise-shell female heterozygous for hair length what kind of kittens would be produced in the F1? If the F1 cats are allowed to generously interbreed among themselves, what are chances of obtain long-h..
DNA sequences differ between species due to substitution events, i.e. when new mutations arise and become fixed by drift or selection in one of the species.
evaluate the melting temperature of the original strand. Because G binds to C more strongly than A binds to T, the melting temperature should increase with the fraction of G/C pairs along the backbone of a sequence.
If one assumes that Hardy-Weinberg equilibrium applies to population of colonists on this planet, about how many people will have attached ear lobes at what time the planet's population reaches 10,000.
Jimmy, a new undergraduate researcher in the lab, is told to make a vector having a copy of the CELLBIO gene from Dr. Acosta's genome as well as a selectable gene, Ampr.
Determine when an organism incorporates genetic material from another orgainism that is not its parent, this is know as
Determine what is the currently accepted scientific explanation for anthropogenic global climate change?
Comparisons of amino acids sequences can shed light on the evolutionary divergence of related species. Would you be expecting all the proteins of a given set of living species to demonstrate the same degree of divergence.
Explain the plant life, animal life, and geology of the ecosystem in the area in which you live. What populations and communities are there? How dependent is your community on this ecosystem? What are the limiting factors of ecosystem.
During glycogenolysis, glycogen is broken down and converted to glucose six phosphate, which can enter glycolysis or be used by the liver to raise blood glucose levels.
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