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RNAse is an enzyme that cleaves the P--O^5' bond in RNA. it has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from ~pH 6.0,
Choose all the true statements
a.) the RNAse reaction is an example of metal ion catalysis with a positively charged metalb.) both His residues are deprotonated at pH higher than 6.5c.) one His residue abstracts a proton from the substrate at pH 6.0, whereas the other denotes a protond.) there are two His residues, and both are deprotonated below pH 4.2e.) the substrate is affected by the change in pH, and is inactive (+ charged) when pH is above ~6.0
In enzyme lab. Manganese Dioxide does not come from a living thing. Is it a catalyst (define catalyst)? Is it an enzyme? Describe. Why did we test the hydrogen peroxide with manganese dioxide.
Would you expect any differences in the sizes of the inserts in the two libraries? in brief explain.
A harder one which requires a little more algebra. You have 90.00 mL of dH20. How much glycerine would you have to add in order to make exactly a 1 percent (v/v) dilution?
A geneticist makes a cross between two homozygous strains of maize with three different mutations. For symbols, you can use a capital to indicate the dominant allele.
Explain normal regulation of blood glucose through the pancreatic hormones. How is this altered with Diabetes?
which of the six types of general reactions they correspond to.
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A biochemist isolates a peptide hormone with the given sequence, Indicte the residues that could contribute a positive charge.
Clostridium and Streptococcus are both catalase-negative. Streptococcus grows by fermentation. Why is Clostridium killed by oxygen, whereas Streptococcus is not.
A cell attempts mitosis with only one spindle pole, but is otherwise normal. Which of the following is true for this cell?
To research the origin of nondisjunction, 200 human oocytes that had failed to be fertilized during in vitro fertilization procedures were examined.
What is a lethal allele? Are lethal alleles usually dominant or recessive? Are there any exceptions? (Hint: Huntington disease in humans). How do we maintain stocks with lethal alleles?
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