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Question 1: Describe DNA sequence variations that are often observed among individuals of the same species. Discuss the possible phenotypic consequences of these DNA sequence variation, give examples, if possible. Discuss the technologies or methods that are often used in detecting each of these variations.
Question 2: Describe the field of epigenetics study. How doepigenetic modifications affect gene expression and phenotype?
Question 3: Briefly describe the principles and applications of 2-dimensional gel electrophoresis, including 2d DIGE, for analysis of proteins.
Question 4: How does real time PCR work to quantify the initial amount of the DNA template? How should the method be modified to quantify the initial amount of RNA template extracted from tissue or cells?
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What do you think would happen if a person had a mutation that prevented the jaw part of their HLA molecules from becoming the correct shape.
A man and woman are both of normal pigmentation and have a one child out of three who is albino (without melanin pigmentation). Albinism is an autosomal (not sex-linked) recessive trait. What are the genotypes of albino's parents.
Think about a population in which the frequency of allele A is p=0.7 and the frequency of allele a is q=0.3, and where the alleles are codominant.
Evaluate the sizes of the different digestive organs of the earthworm. Which organ is largest in the earthworm? How does its size make it well suited for its function.
If you wanted 10mL of a 1mM nitrophenol solution and you have on hand a 50mM nitrophenol solution, how would you make it. If you wanted 100mL of a 0.1M acetic acid solution, how much 17.4M acetic acid and how much water do you need to add to make t..
Life arose in water. Explain w hy do you think colonization of land represented a major evolutionary step in the history of life?
What is an advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein.
In a testcross of Aa Bb CC Dd Ee FF where the genes demonstrate independant assortment and determine the expected frequency of aa bb Cc dd ee Ff progeny?
Find out the genotypes of the two black females and the brown male using allele symbols B and b. One shows linear carbon chains, another has one -CH3 groups present on the third carbon atom, and third has two -CH3 groups present on the second carbon ..
Assume you have an unknown DNA that you know is phage DNA - It is either T7 or lambda. You perform a restriction enzyme digest and run the results on a gel.
The top of mountain is at an elevation of 45° from one bank of a river and at an elevation of 60° from the other bank. If river has a width of 1 km, what is the height [in km] of the mountain.
Why have both an endocrine and a nervous system? however we have not yet studied the endocrine system, you know enough about it to answer the question.
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