Describe how monoclonal antibodies are produced

Assignment Help Biology
Reference no: EM1398305

A project is underway to develop a vaccine against mouse hepatitis virus, a pathogen of mice. The vaccine consists of capsid protein of the virus, which is injected subcutaneously as a depot in alum on day 0. At days 5 and 14, the group of six mice is bled and the serum is tested for the presence of antibodies against the viral capsid protein. Separate assays are done for each of the immunoglobulin classes, IgM, IgG and IgA. The amounts, expressed in ?g/ml of antibody, are shown in the figure below. When the data is analysed it appears that two of the animals (asterisks) have high titres of antibody particularly IgG and IgA, at both days 5 and 14. a) Why do the titres of IgG antibodies increase more rapidly between days 5 and 14 than do IgM antibodies, in all animals? b) Propose an explanation for the high titres of IgG antibodies in the two animals indicated at day 5. Can this explanation also account for the relatively high levels of IgA antibodies also seen in these mice? (Note: Mouse hepatitis virus infections can be common in animal houses) The spleens from mice taken at day 14 are used to produce B cell hybridomas making monoclonal antibodies against the viral protein. Of the clones produced, 15 produced IgG, three produce IgM and none produced IgA. c) Why do you suppose there are no IgA-producing clones, despite the good IgA response ? d) You want a high-affinity antibody for use in an assay. Which of the clones you have produced are likely to be of higher affinity? e) Describe how monoclonal antibodies are produced.

Reference no: EM1398305

Questions Cloud

Explain the main trends in tfr and replacement fr : please explain the main trends in TFR and replacement FR in developed versus devloping countries? How can TFR of the overall human population be lowered.
Predicted value of cricket chirps : Find the best predicted temperature (in degree F) at a time when a cricket chirps 3000 times in one minute. What is wrong with this predicted value?
What is the frequency of w in this genetics class : The ability to taste "Wine" is controlled by a dominant allele W, while individuals homozygous for the recessive allele w, are unable to taste Wine. In a recent genetics class of 125 students, 88 were able to taste Wine, but 37 could not. Assume H..
Which direction of research appears to having most success : which direction of research appears to be having the most success.
Describe how monoclonal antibodies are produced : A project is underway to develop a vaccine against mouse hepatitis virus, a pathogen of mice. The vaccine consists of capsid protein of the virus, which is injected subcutaneously as a depot in alum on day 0. At days 5 and 14, the group of six mic..
Recent business trip for an internal company visit : What is the probability that more than 25% of the business travelers say that the reason for their most recent business trip was an internal company visit?
What is mrs. x red blood cell volume after the infusion : What is Mrs. X red blood cell volume after the infusion equilibrates with the blood and why? Also what is the osmolarity of the red blood cells and plasma AFTER equilibration.
Which one of the following statements is true : Which one of the following statements is true.
Normal lac operon in the presence of lactose and glucose : An enzyme is discovered that selectively shortens telomeres. Its gene is isolated, cloned and transfected into a malignant cell line where it is transcribed and translated.

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd