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E. coli were transformed with 50 ng of plasmid DNA is a total volume of 500 µl, and 100 µl of the transformation reaction was plated on a LB amp agar plate. After overnight incubation at 37C.
Count the colonies and calculate the transformation efficiency for this experiment.
Analysis of preserved leaves shows that the density of stomata per unit area has decreased over the past 200 years. Suggest (and support) a hypothesis that explains this phenomenon.
An eight year old boy has an Individualized Education Plan due to learning problems. When he reads, he frequently misses letters and occasionally entire words.
Red pigment is produced in a fictional plant through the action of the gene paintball (P). Flowers of varying intensity from pale pink to bright red are made when the activity of the paintball enzyme is between 24 and 50.
Meyer and Overton, at the turn of the last century, conducted a seminal study on the influence/efficacy of anesthetics on cell membrane fluidity. Briefly explain their findings in the context of biochemistry.
Describe the detailed structure of DNA, and explain the manner in which this structure replicates itself in both prokaryotes and eukaryotes.
Two dissimilar inbred strains of mice, A and B, are crossed to yield an F1 hybrid strain, AB. Suppose if a large dose of spleen cells from an adult A mouse is injected into an adult AB mouse,
Why cant microbes that naturally clean up the environment continue to do so as in the past? How have humans made their task harder? Easier?
Determine which of the following pairs of functional groups CANNOT react with each other by a dehydration reaction?
A mammal uses only 1 to 2 percent of its energy in ventilation while a fish must spend about 15 percent of its energy to move water over its gills. Describe this huge difference in their efforts to collect oxygen.
Was there any noticeable effect on the germination rate of the radish seeds as a result of the pH? Compare and contrast the growth rate for the control with the alkaline and acidic solutions.
Suppose that a dihybrid cross is made in which gene loci are autosomal, independently assorting, and incompletely dominant.
Suppose that you have come up with a way of enlarging single cells to such a degree that a DNA duplex has a diameter of 0.1 mm - about the thickness of a human hair.
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