"PART- B1. Describe how did you set up the Y-site assembly to collect a sample containingamoxicillin, parenteral nutrition (PN) and lipid that could be used to investigate thechemical and physical incompatibility of amoxicillin, PN and lipid. In addition, using asuitable clinical example discuss - why administration of total parenteral nutrition (TPN- PN +lipid) and anidulafungin would be required to a patient using a Y-site assembly.Approximately 250 to 300 words2. A. In week 2, you were provided with the samples containing amoxicillin, PN andlipid. The concentrations of amoxicillin, PN and lipid were calculated based on the dosesof amoxicillin (infused over a period of 30 minutes) and TPN (infused over a period of 24hours) used in 0.5kg (Sample A) and 5kg (Sample B) patients. The samples were thensubjected to centrifugation. Report the results obtained after centrifugation. If theresults obtained from the two samples (prepared based on 0.5 and 5kg body weights)were different from each other then discuss the potential reasons for this difference.Approximately 150 to 200 wordsB. In week 2, lipid free sample containing amoxicillin, PN and water was prepared. Theconcentrations of amoxicillin and PN were calculated based on the doses of amoxicillinand TPN used in 5 kg (Sample C) patients. The sample was then subjected for visual andmicroscopy analyses. Report and DISCUSS the results obtained after these analyses.Your discussion should also include whether the results obtained after visual andmicroscopy analyses (Sample C) were different from the results obtained aftercentrifuging the lipid-containing sample (B).Approximately 150 to 200 wordsC. In week 3, you prepared a lipid free sample (either sample D, E or F) containingflucloxacillin, PN and water. The concentrations of flucloxacillin and PN in the samplewere calculated based on the doses of flucloxacillin and TPN used in either 0.5kg(Sample D), 3kg (Sample E) or 5kg (Sample F) patients. Report and discuss the resultsobtained after these analyses. In your discussion, you are required to discuss - why theresults obtained from the analyses of three samples (D, E and F) were similar and/ordifferent from each other.Approximately 150 to 200 wordsD. In week 4, a lipid free sample containing amoxicillin, PN and water was prepared.The concentrations of amoxicillin and PN were calculated based on the doses ofamoxicillin (infused over a period of 24 hours) and TPN (infused over a period of 24hours) used in 0.5 kg (Sample E) patients. Microscopic and visual analyses of the sampledid not show the signs of precipitation or particles.Answer the following questions:1. Briefly describe the methodology you used to prepare the lipid free sample.2. In clinical practice, would it be appropriate to infuse amoxicillin over a period 24hours (also referred to as continuous infusion)?3. Which one of the two types of amoxicillin infusion (1- continuous and 2 - intermittentinfusion; e.g. three times a day) has a better clinical efficacy? Support your answer using a suitable example.Approximately 150 to 250 words3. Why is it important to evaluate the pH of an admixture (antibiotic-PN-lipid) collectedat y-site? Support your answer with at least two suitable examples.Approximately 200 to 300 words4. Briefly describe the important limitation of UV spectrophotometer in determining thenumber of particles present in an admixture (3ml - containing amoxicillin, PN and lipid)collected at Y-site.1) Describe how did you set up the Y-site assembly to collect a sample containingamoxicillin, parenteral nutrition (PN) and lipid that could be used to investigate thechemical and physical incompatibility of amoxicillin, PN and lipid. In addition, using asuitable clinical example discuss - why administration of total parenteral nutrition (TPN– PN +lipid) and anidulafungin would be required to a patient using a Y-site assembly?Approximately 250 to 300 wordsThe Y site assembly consists of the following parts,? Infusion line? 0.2 micron and 1.2 micron filter? Amber colored extension line for lipid ? Drug infusion line? Y site connector line? Extension set for the lipid and drug administration? Sample collection point?The total parenteral nutrition (saline) is held at a height above the ground against the gravity,the infusion line from the TPN is passed through the machine, by which the flow rate of TPNis appropriately fixed and pall nano dyne NEO filter 0.2-micron filter is attached just beforethe Y site connector. Two extension sets are present one for the lipid or water and other for the drug administration,amber colored extension line for the flow of lipid from lipid extension set to the Y siteconnector. In the amber colored line before connecting to the Y site connector, 1.2 micronfilter is connected to capture particles the particles present in the lipid sample.The other extension set for the drug administration is connected from the machine to the Ysite without any filter. All the extension lines from all the 3 different sources are connected tothe Y site connector, where the TPN, lipid and the drug are mixed together, before being sentto the collection point, where the sample is collected for the physical and chemical studies.2) A.In week 2, you were provided with the samples containing amoxicillin, PN andlipid. The concentrations of amoxicillin, PN and lipid were calculated based on the dosesof amoxicillin (infused over a period of 30 minutes) and TPN (infused over a period of 24hours) used in 0.5kg (Sample A) and 5kg (Sample B) patients. The samples were thensubjected to centrifugation. Report the results obtained after centrifugation. If theresults obtained from the two samples (prepared based on 0.5 and 5kg body weights)were different from each other then discuss the potential reasons for thisdifference?Approximately 150 to 200 words.0.5 kg and 5 kg body weight samples were prepared and were subjected to centrifugation for20 minutes and inspected for the presence of particles against light. The sample preparedaccording to the 0.5 kg body weight showed the presence of precipitate and the 5-kg sample had not showed the presence of it and the sample with the presence of precipitate wasdiscarded. I believe the presence of particles was due to presence of more quantity of totalparenteral nutrition than the drug, as the TPN consists of calcium, magnesium and other saltswhich can lead to the formation of precipitate.For 0.5 Kg the volume of drug (Amoxicillin) used was 0.306 ml for 1 hour, volume of lipidwas 0.122 ml for 1 hour and TPN was 2.571 ml for 1 hour. For 5 Kg body weight the volumeof drug used was 0.77 ml for 1 hour, lipid was 0.29 ml/hour and the volume of TPN used was1.932 ml/hour.B. In week 2, lipid free sample containing amoxicillin, PN and water was prepared. Theconcentrations of amoxicillin and PN were calculated based on the doses of amoxicillinand TPN used in 5 kg (Sample C) patients. The sample was then subjected for visual andmicroscopy analyses. Report and DISCUSS the results obtained after these analyses.Your discussion should also include whether the results obtained after visual andmicroscopy analyses (Sample C) were different from the results obtained aftercentrifuging the lipid-containing sample (B)? Approximately 150 to 200 words.Lipid free sample for 5 Kg body weight was prepared by adding water in the place of lipid,PN and drug they were visually inspected for the presence of particles and the absence ofparticles was found out by visual inspection method and in order to confirm the presence ofparticles microscopical analysis was performed.For confirming the presence of particles, both positive (with particles) and negative control(with no particles) samples were prepared. When both the samples were subjected tomicroscopical analysis, the presence of particles was confirmed by comparing the identifiedobjects with the particles present in the positive control. For the lipid containing samples for "