Large-scale and continuous culture Assignment Help

Assignment Help: >> Microbiology - Large-scale and continuous culture

arge-scale and continuous culture

A flexible large-scale batch culture unit has been established for the growth of plant cell suspension cultures. This unit has been changed to permit of continuous or intermittent renewal of culture medium and in a modified form incorporated into open continuous culture systems of the turbidostat and chemostat kind. A fully automatic culture sampler has been incorporated into the simple culture unit.


 If a stirred tank reactor is fed with fresh medium at a constant rate and excess is allowed to flow to waste a culture will be created with constant growth rate. This is known as steady-state culture. If limited through carbon source this is called a chemostat, but biomass and redox variations are possible. The constant and defined nature of the culture allows the calculation of many parameters including replacement time, maximum growth rate or biomass and growth-limiting substrate concentrations.

 

                                            367_Large-scale and continuous culture.png

 

The Continuous culture studies were initiated through us for two purposes. The first was to establish a better understanding of the regulatory mechanisms involved in the biosynthesis of alkaline protease neutral protease and a-amylase through Bacillus subtilis NRRL-~341 me. This information is  fundamental  to understanding how  to  alter  these mechanisms  in  order  to  give  a desired kind of micro-organism.


Shake-flask batch culture provides a simple and convenient technique of growing small amounts of microorganisms. However, if grams of biomass are needed for protein purification or liters of medium are needed for product recovery then the limitations of the shake flask quickly become apparent. The  most significant problem is getting enough oxygen  to the  culture: to grow  a 5 liter  culture in a single  flask would require a 50 liter glass Erlenmeyer flask if the  ratio  of one-tenth volume of culture to the  total  volume of container is maintained. Furthermore the shake flask is a highly dynamic system. As the organism grows it excretes primary and secondary metabolites into the medium. Some of these metabolites might actually prevent efficient use of the substrate but more significantly there may be a change in pH by many units. If a batch culture of 1 liter or more is to be grown efficiently more controlled conditions are required.

Continuous culture Other fermenter types
The simple stirred tank fermenter
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd