Yersinia enterocolitica, Biology

Yersinia enterocolitica

The genus Yersinia contains of 3 human pathogenic species, Y. enterocolitica, Y. pseudotuberculosis and Y. pestis, among which, Y. enterocolitica finds a major importance in food-borne cases.

Epidemiology: Yersinia are gram-negative straight rods or small cocci (0.5-0.8µm) and non-motile at 37°C but motile at temperature below 30°C. They are facultative anaerobic and do not produce H2S. They can grow at temperatures as low as 0oC, and the increased use of refrigeration in food chain may play a role in the increase in prevalence of these organisms. Different serotypes of Y.enterocolitica are associated with human infections in different regions of the world. As with other foodborne illnesses, the infection is transmitted by faecal-oral route. Toxin produce.


Clinical features:
Gastroenteritis or mesenteric adenitis are the important clinical features. Severe abdominal pain, diarrheoa, fever; blood in stool; polyarthritis, erythema nodosum, especially in children is seen.


Diagnosis:
Y. enterocolitica being psychrophilic, the sample is inoculated into a non-selective medium such as 1/15 M PBS and incubated at 4 °C for 21 days. Alternatively, selective enrichment broth such as bile oxalate sorbose (BOS) broth, irgasan ticarcillin chlorate (ITC) broth, peptone-sorbitol-bile salt broth (PSB) may be used at incubation temperature from 22°C to 25°C for fewer days (2-5 days). Selective media used are cefsulodin irgasan novobiocin agar (CIN) on which red bulls eye colonies appear. Virulent Yersinia enterocolitica agar (VYE) showing red colony, Salmonella-Shigella deoxycholate calcium chloride agar (SSDC) and MacConkey agar showing small, round colorless colonies are also used for isolation of Y. enterocolitica. The plasmid (PYV) along with heat stable enterotoxin gene yst, the invasive associated ail and inv and virF gene are used for the detection of virulence Y. enterocolitica by
PCR methods.

Posted Date: 9/20/2012 2:52:43 AM | Location : United States







Related Discussions:- Yersinia enterocolitica, Assignment Help, Ask Question on Yersinia enterocolitica, Get Answer, Expert's Help, Yersinia enterocolitica Discussions

Write discussion on Yersinia enterocolitica
Your posts are moderated
Related Questions
Explain the term 'health' Mention any two ways of maintaining it. Why does a doctor administer tetanus antitoxin and not a tetanus vaccine to a child Injured in a roadside acci

How do taenias classify according to the division of sexes? Taenias are monoecious (hermaphrodite), the similar individual has female and male reproductive organs and undergoes

IMPORTANCE OF MENDELISM 1 .       Improvement of Plants Hybridization is used for obtaining improved varieties of plants. This process results in combinations of desirable

What capacity does the brain have to repair itself after injury or disease? It has long been accepted dogma that central nervous system doesn't regenerate itself after traumati

Describe the significance of micronucleus. One of two types of dimorphic nuclei found in ciliate protozoans. The single micronucleus contains only one copy of the genome and is

Vitamin D (Calciferol - vitamin D2) The main forms are Vitamin D2 (ergocalciferol-plant origin) and vitamin D3 (cholecalciferol-animal origin). Vitamin D2 forms colourless, aci

Hormone s . Cortical steroids (corticoids-hormones of adrenal cortex) are grouped into three catagories : mineralocorticoids, glucocorticoids and gonadocorticoids. (i) Mine

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

During the course of a physiology laboratory, a student finds that her PR interval is 0.24 second. Concerned, she takes her own ECG again an hour later and sees an area of the ECG

Write about embryonic stem cell invitro methord in which transgenic animal were generated and efficient show how homologous recombination happening?