Yersinia enterocolitica, Biology

Yersinia enterocolitica

The genus Yersinia contains of 3 human pathogenic species, Y. enterocolitica, Y. pseudotuberculosis and Y. pestis, among which, Y. enterocolitica finds a major importance in food-borne cases.

Epidemiology: Yersinia are gram-negative straight rods or small cocci (0.5-0.8µm) and non-motile at 37°C but motile at temperature below 30°C. They are facultative anaerobic and do not produce H2S. They can grow at temperatures as low as 0oC, and the increased use of refrigeration in food chain may play a role in the increase in prevalence of these organisms. Different serotypes of Y.enterocolitica are associated with human infections in different regions of the world. As with other foodborne illnesses, the infection is transmitted by faecal-oral route. Toxin produce.


Clinical features:
Gastroenteritis or mesenteric adenitis are the important clinical features. Severe abdominal pain, diarrheoa, fever; blood in stool; polyarthritis, erythema nodosum, especially in children is seen.


Diagnosis:
Y. enterocolitica being psychrophilic, the sample is inoculated into a non-selective medium such as 1/15 M PBS and incubated at 4 °C for 21 days. Alternatively, selective enrichment broth such as bile oxalate sorbose (BOS) broth, irgasan ticarcillin chlorate (ITC) broth, peptone-sorbitol-bile salt broth (PSB) may be used at incubation temperature from 22°C to 25°C for fewer days (2-5 days). Selective media used are cefsulodin irgasan novobiocin agar (CIN) on which red bulls eye colonies appear. Virulent Yersinia enterocolitica agar (VYE) showing red colony, Salmonella-Shigella deoxycholate calcium chloride agar (SSDC) and MacConkey agar showing small, round colorless colonies are also used for isolation of Y. enterocolitica. The plasmid (PYV) along with heat stable enterotoxin gene yst, the invasive associated ail and inv and virF gene are used for the detection of virulence Y. enterocolitica by
PCR methods.

Posted Date: 9/20/2012 2:52:43 AM | Location : United States







Related Discussions:- Yersinia enterocolitica, Assignment Help, Ask Question on Yersinia enterocolitica, Get Answer, Expert's Help, Yersinia enterocolitica Discussions

Write discussion on Yersinia enterocolitica
Your posts are moderated
Related Questions
Suppose the following base sequence was found in a 21 base DNA polymer. 3' TAC   TTA   GGC   TCC    GTT    CAG   ATT       5' 1. Provide the nucleotide sequence complementary

The Carbohydrate sets are covalently attached to various variant proteins to form glycoproteins. Carbohydrates are a shorter percentage of the weight of glycoproteins than of prote

Tabulate macro & micro nutrients on the basis of -elements, source, synthesis site, symptoms

What do you understand by Coelenteron? The name given to internal cavity of the cnidarians. This is an incomplete gut with just one opening, the mouth. Food to be digested and

Your task is to describe genetic algorithms, explain why genetic algorithms are useful in solving the problem you have been set and conduct an investigation into the optimum parame

Generalised Seizures Generalised seizures are bilaterally symmetrical without focal onset. One subtype, the grand mal attack, is characterised by loss of consciousness and by s

Uses of modified starches in food and confectionery industries Visit a grocery shop and look out for products such as the instant desserts, jelly beans, salad dressings, tomato

Define Laser Tweezers Technology? In addition to above said classical methods, advance technologies can be used for obtaining pure cultures. Laser tweezers technology is useful

Explain the Phylum Molluska - Mollusks? You are probably very familiar with members of this phylum. The mollusks include the octopuses, squids, cuttlefish, scallops, oysters, c

Wildlife - Ecology The term wildlife probably originated in 1913 in a book, Our Vanishing Wildlife by William Hornaday, Director of the New York Zoological Park. The main focu