What the two pcr amplified strands, Biology

You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed.

2129_exon.png

(a)  Design PCR primers (indicate regions of primers with black, blue and red colours by sketching on the plasmid map) for a PCR approach that could result in the deletion of the intron from this gene so that it can be expressed in the resulting plasmid in E. coli.

(b)  Sketch what the two PCR amplified strands would look like from the parental plasmid,

Sketch what the subsequent two PCR amplified strands would look like from newly synthesised DNA [DNA strands indicated in (b)].

Posted Date: 3/11/2013 1:37:44 AM | Location : United States







Related Discussions:- What the two pcr amplified strands, Assignment Help, Ask Question on What the two pcr amplified strands, Get Answer, Expert's Help, What the two pcr amplified strands Discussions

Write discussion on What the two pcr amplified strands
Your posts are moderated
Related Questions
Insects - Hormones in Growth and Reproduction In insects hormones regulate moulting and metamorphosis. The larvae or nymphs which hatch out of the eggs undergo regular moultin

Basic structural pattern of limbs The common structural pattern of tetrapod limbs is extremely clear expressed in their skeletal components and in the manner they are organiz


Talk about briefly three  of the common enteric diseases caused by inadequate clean water supply and insufficient sanitation facilities. Your talk should contain the source and spr

Describe Infectious Agents ? Recently there has been increasing interest in the relation between chronic low grade systemic inflammation and mortality of coronary artery diseas

Q. What is the structure that to maintains identical chromatids bound? The structure that to maintains identical chromatids bound is the centromere.

POULTRY BREEDING Poultry breeding has acquired pre-eminence over all other domesticated livestock enterprises. Production level of layer and broiler type of chicken touched spe

Q. Why is it significant for chromosomes to be condensed during mitosis and decondensed during interphase? During mitosis the major problem to be solved is the correct separati

It consists of: - Two kidneys that produce urine. - The left and right ureters that the urine travels through on leaving the kidneys. - The muscular urinary bladder, which

Q. What is the main molecule responsible for the absorption of photic energy for photosynthesis? And where is that molecule located in photosynthetic cells? The chlorophyll mol