What the two pcr amplified strands, Biology

You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed.

2129_exon.png

(a)  Design PCR primers (indicate regions of primers with black, blue and red colours by sketching on the plasmid map) for a PCR approach that could result in the deletion of the intron from this gene so that it can be expressed in the resulting plasmid in E. coli.

(b)  Sketch what the two PCR amplified strands would look like from the parental plasmid,

Sketch what the subsequent two PCR amplified strands would look like from newly synthesised DNA [DNA strands indicated in (b)].

Posted Date: 3/11/2013 1:37:44 AM | Location : United States







Related Discussions:- What the two pcr amplified strands, Assignment Help, Ask Question on What the two pcr amplified strands, Get Answer, Expert's Help, What the two pcr amplified strands Discussions

Write discussion on What the two pcr amplified strands
Your posts are moderated
Related Questions
Conduction An action potential occurs at one point along the axon. Yet we know that neurological impulses are not fixed, they travel along a neuron. So how can the action pote

Prostaglandins Prostaglandins belong  to a subclass of  lipids known  as the eicosanoids because of their structural similarities to  the C-20  polyunsaturated  fatty acids, t

What is monera? One of the 5 main kingdoms contains bacteria and blue/green algae. Does NOT have a cell membrane, or in other words, is made of prokaryotic cells. Actually,

Explain several biological classification of animals There are several biological classification of animals. There are: i) Phenetic Classification ii) Natural Classificati

Characteristic Features of Avian Gastrulation Creation of hypoblast and its important role in formation of the axis and orientation of embryo. Existence of the cells

Cohesion-adhesion theory describes the properties of water which help move it through the plant. Cohesion is the property of water molecules to stick together (held by hydrogen bo

Explain Technical factors influencing food production? Improvement in technology has a significant impact on productivity. Improvements may occur in seed production, fertilizer

Explain Chemical Structure of saponins? Let us briefly understand their chemical nature. Their structures are characterized by the presence of a steroid or triterpene group, i.

Q. Concerning their biological function what is the difference between meiosis and mitosis? The main biological function of mitosis is cellular multiplication a fundamental pro

questions and answers on human impacts on environment