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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
Explain about the Vacuum Evaporators? It is common to construct various vacuumised vessels in series that is why the product moves from one vacuum chamber to the next and thus
what are the character selection criteria?
migration
Explain the effect of Deficiency of pyridoxine? A deficiency of vitamin B6 alone is uncommon because it usually occurs in association with a deficit in other B-complex vitamin
Explain acid and bases? Acids and Bases : Some aqueous solutions, however, such as HCl, form an excess of hydrogen ions [ H + ] and are called acids. Others, such as NaOH,
What are lateral meristems? Where can they be found and which type of plant growth do they promote? Lateral, or secondary, meristems, are the cambium and the phellogen (also ca
Difference between rods and cones - Rods Cones 1. More in number 1150 lakh 2. Outer seg. is cylindrical and contains
Q. Symptoms and Complications of atherosclerosis? Symptoms Excessive weight, hypertension, high levels of cholesterol and triglycerides. Complications Myocardia
Q. Define the Organization Levels of Life? Ans. The cell is the basic unit of life - the smallest unit that can carry on all the processes of life. Organisms that consist of a
Simple diffusion Only comparatively small uncharged or hydrophobic molecules (H2O, O2, and CO2, other gases, urea and ethanol) cross the lipid bilayer through simple
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