Vital dye staining, Biology

Vital Dye Staining

In this type of method, a piece of agar or cellophane soaked in the solution of a vital dye (Neutral Red, Nile Blue Sulphate, and Bismarck Brown etc.) is applied to the surface of embryo at the needed position. The dye diffuses from agar (or cellophane) to the cells of the embryo at that position. The embryo carries on to develop normally. The stained blastomeres and their descendent cells take the dye for quite a long time and there is little or no diffusion from them to non-stained cells. The final position of stained cells in the later differentiated embryo points out the fate of the originally stained blastomeres. With this type of method the detailed fate maps of amphibian blastula and some other embryonic stages have been made.

 

Posted Date: 1/25/2013 4:39:26 AM | Location : United States







Related Discussions:- Vital dye staining, Assignment Help, Ask Question on Vital dye staining, Get Answer, Expert's Help, Vital dye staining Discussions

Write discussion on Vital dye staining
Your posts are moderated
Related Questions
The aqueous solution with the lowest pH is: Select one: a. 0.01 M HCl. b. 0.1 M acetic acid (pKa = 4.86). c. 0.1 M formic acid (pKa = 3.75). d. 0.1 M HCl. e. 10 t

For many of the mammalian Hox genes, it has been possible to determine that some of them are more similar to one of the insect HOM-C genes than to the others. Describe an experimen

R a d i o g raphic imaging: Radiology has been in use since the discovery of X-rays by Wilhelm Conrad Roentgen in 1895. Until 1980s, radiography was the

Genital warts and human papillomavirus (hpv) infection External genital warts are caused by human papillo- mavirus, usually type 6 or 11; other types (16, 18 and others) cause

how do carnivorous feed

Post removal Technique using PRS kit -Transmetal efficiently doming the post head . -Select suitable sized trephine . -Place drop of RC prep on the post head as lubrican

What are the Risks of migration Eg Getting lost (blown off course or similar) and the effect of this when there is a limited fuel supply. Insufficient food to build

Q. Evaluation of osseointegration? All the parameters of proper implant selection, atraumatic sequential osteotomy preparation and the attainment of primary stability are focus

Ask queAffinities of ctenophora with platyhelminthesstion #Minimum 100 words accepted#

Explain Metabolism of Iron? In this sub-section, we will study how body gets its iron supply, how iron is transported and utilized by the various tissues and how iron balance i