Viral vectors, Biology

Viral vectors  

There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows:

  • Adenoviral vectors:  They are most widely used vectors which are capable or transferring large DNA inserts (up to 35 kilo base pairs). Adenoviruses are human viruses and hence can transduce a large number of human cells efficiently. They have been used in diseases like cystic fibrosis (Anderson, N.D). Their capacity to infect quiescent and dividing cells and producing high-titre viral stocks has increased their application (Gould and Favorov, 2003).
  • Adeno-associated viral vectors: This non pathogenic mammalian virus shows a high prevalence within human beings. It specifically integrates into the short arm of the human chromosome and does not produce diseases (Anderson, N.D). As the genome of AV is small it allows the transfer of about 4.8 kb of DNA. This vector has been used during the experimental studies of diabetes and arthritis.
  • Herpes simplex virus: A large part of the long genome of HSV (150 kb) can be replaced with a foreign DNA. This does not effect the viral growth and allows the transfection of multiple genes. They have the capacity to transduce nervous cells and other cell types also. The infection of cells by HSV is a complex process which involves the interaction between viral glycoproteins and proteoglycans of the host cell surface (Gould and Favorov, 2003).
  • Retroviral vectors: The retroviral genome is linear stretch of RNA which is 7-11 kb long. General components of the retroviral genome are two LTRs, located at the terminal positions of the genome and cis-signals lie adjacent to LTRs. These cis-signals are responsible for integration and reverse transcription. These regions are utilized during the designing of the retroviral vectors (Gould and Favorov, 2003).
Posted Date: 8/9/2012 6:35:32 AM | Location : United States

Related Discussions:- Viral vectors, Assignment Help, Ask Question on Viral vectors, Get Answer, Expert's Help, Viral vectors Discussions

Write discussion on Viral vectors
Your posts are moderated
Related Questions
A patient was complaining of frequent urination, excessive thirst and dehydration. His fasting glucose level was found to be normal. Name the disease and its cause. Describe

1.   Carbon monoxide (CO): It is colourless, odourless, tasteless gas and is not soluble in water. Source: CO is produced due to: (i)     Incomplete combustion of fuels

Maedi/visna Maedi, a progressive pneumonia, and visna, a neurotropic disease, found in sheep and goats are caused by the Maedi/visna virus, belonging to the family Retroviridae

CONCEPT OF NURSING UNIT: Concept in the design and facilities of nursing unit has been changed time to time based on the different categories of patients and peculiarities of

Primary mediators are those, which are produced before degranulation. These primary mediators are stored in granules. Some of the primary mediators are histamine, heparin, protease

Why there is difference in the colour obtained for the dextrin solution (ammonium sulphate and dextrin )when brought to iodine test Respetively by first half saturation test and t

Q. What is meant by unsaturation or saturation of oils and fats? When it is said that a triglyceride is saturated it means that in its molecule the carbon chain is bound in its

You work with flies and you have identified a single gene that can cause flies to either have two heads or one head (depending on the genotype of this gene), and that is all you kn

ae there non-parasitic viruses?