Viral vectors, Biology

Viral vectors  

There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows:

  • Adenoviral vectors:  They are most widely used vectors which are capable or transferring large DNA inserts (up to 35 kilo base pairs). Adenoviruses are human viruses and hence can transduce a large number of human cells efficiently. They have been used in diseases like cystic fibrosis (Anderson, N.D). Their capacity to infect quiescent and dividing cells and producing high-titre viral stocks has increased their application (Gould and Favorov, 2003).
  • Adeno-associated viral vectors: This non pathogenic mammalian virus shows a high prevalence within human beings. It specifically integrates into the short arm of the human chromosome and does not produce diseases (Anderson, N.D). As the genome of AV is small it allows the transfer of about 4.8 kb of DNA. This vector has been used during the experimental studies of diabetes and arthritis.
  • Herpes simplex virus: A large part of the long genome of HSV (150 kb) can be replaced with a foreign DNA. This does not effect the viral growth and allows the transfection of multiple genes. They have the capacity to transduce nervous cells and other cell types also. The infection of cells by HSV is a complex process which involves the interaction between viral glycoproteins and proteoglycans of the host cell surface (Gould and Favorov, 2003).
  • Retroviral vectors: The retroviral genome is linear stretch of RNA which is 7-11 kb long. General components of the retroviral genome are two LTRs, located at the terminal positions of the genome and cis-signals lie adjacent to LTRs. These cis-signals are responsible for integration and reverse transcription. These regions are utilized during the designing of the retroviral vectors (Gould and Favorov, 2003).
Posted Date: 8/9/2012 6:35:32 AM | Location : United States







Related Discussions:- Viral vectors, Assignment Help, Ask Question on Viral vectors, Get Answer, Expert's Help, Viral vectors Discussions

Write discussion on Viral vectors
Your posts are moderated
Related Questions
Q. Nutritional Management for lactose intolerance? Diagnostic tests are available that can give information about the level and activity of the lactase enzyme. Depending on the

why sporophyte of psilopsida is rootless

Known physicochemical basis of some of these texturization processes are presented below: 1.Thermal Coagulation and Film Formation: Concentrated soy proteins can be thermally

Anomalies Related to Oral Cavity: Under these anomalies we will briefly discuss the cleft lip and cleft palate.  You must have seen and/or nursed a baby born with a cut on

extracellular signals and their characteristics

A saline drip is to be prepared for a lab experiment that is looking at hydration in lab rats. Calculate the amount of NaCl need to make 4 liters of a 0.9% solution.

Explain factors influencing rs content of foods? 1) Presence of sugar and lipids: Studies so far show that presence or addition of lipids and sugar reduces RS content. 2) Ca

Explain the Catabolic Responses? Hormonal responses during the hyper inetabolic phase of infection are same as in case of injury. Serum cortisol levels are elevated, glycogen i

Define Heart Diseases - excessive fat intake? Heart diseases show strong links to fat and cholesterol intake. Foods rich in cholesterol are of animal origin and given in Table.

describe each and every step in nutrition in animals?