Viral vectors, Biology

Viral vectors  

There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows:

  • Adenoviral vectors:  They are most widely used vectors which are capable or transferring large DNA inserts (up to 35 kilo base pairs). Adenoviruses are human viruses and hence can transduce a large number of human cells efficiently. They have been used in diseases like cystic fibrosis (Anderson, N.D). Their capacity to infect quiescent and dividing cells and producing high-titre viral stocks has increased their application (Gould and Favorov, 2003).
  • Adeno-associated viral vectors: This non pathogenic mammalian virus shows a high prevalence within human beings. It specifically integrates into the short arm of the human chromosome and does not produce diseases (Anderson, N.D). As the genome of AV is small it allows the transfer of about 4.8 kb of DNA. This vector has been used during the experimental studies of diabetes and arthritis.
  • Herpes simplex virus: A large part of the long genome of HSV (150 kb) can be replaced with a foreign DNA. This does not effect the viral growth and allows the transfection of multiple genes. They have the capacity to transduce nervous cells and other cell types also. The infection of cells by HSV is a complex process which involves the interaction between viral glycoproteins and proteoglycans of the host cell surface (Gould and Favorov, 2003).
  • Retroviral vectors: The retroviral genome is linear stretch of RNA which is 7-11 kb long. General components of the retroviral genome are two LTRs, located at the terminal positions of the genome and cis-signals lie adjacent to LTRs. These cis-signals are responsible for integration and reverse transcription. These regions are utilized during the designing of the retroviral vectors (Gould and Favorov, 2003).
Posted Date: 8/9/2012 6:35:32 AM | Location : United States







Related Discussions:- Viral vectors, Assignment Help, Ask Question on Viral vectors, Get Answer, Expert's Help, Viral vectors Discussions

Write discussion on Viral vectors
Your posts are moderated
Related Questions
Q. Symptoms of ulcerative colitis? As discussed in the case study above, the common symptoms are: 1. Mild abdominaI discomfort, an urgent need to defecate several times a da

Considering the nature of negative-feedback control and the function of the respiratory system, what effect do you predict that a decrease in CO2 in the internal environment would

Q. Enumerate the various physical agents of sterilization, and list the advantages of moist heat in sterilization process Various physical agents of sterilization are Sun

Where in the cell can ribosomes be found? What is the main biological function of ribosomes? Ans) Ribosomes can be found free in the cytoplasm, adhered to the outer side of the

Q. Medical and Nutritional Management for pectic ulcer? To provide physiological rest and support tissue healing, treatment should be based on providing rest to the affected ar

What is Multiple Alleles ? Obviously, diploid organisms, such as humans can have only two different alleles for a certain gene locus. However, new alleles are continuously bein


Limitations of Intra Oral Periapical Radiographs  It is not optimal in pre operative planning of cases with severely resorbed ridges  Anatomical structure of importance can'

Hazards Is an event whether natural or man-made that has potential for causing injury, loss of life and damage to the property or the environment. It can be grouped into two broad

Q. Can the amount of available energy in a given trophic level be larger than the available energy in inferior trophic levels? What does that condition means to the conformation of