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The rRNA transcription units in E. coli hold some tRNA genes which are processed and transcribed at the time of rRNA transcription. The other tRNA genes happen in clusters of up to seven tRNA sequences separated by spacer regions. Subsequent transcription by the one prokaryotic RNA polymerase, the
Figure: Transcription and processing of tRNA in prokaryotes.
mainly RNA transcript folds up into the characteristic stem-loop structures and is then processed in an ordered series of cleavages by ribonucleases (RNases) that release and trim the tRNAs to their final lengths. The trimming and cleavage reactions at the 5' and 3' ends of the precursor tRNAs contained RNases D and E, F and P. RNases E, F and P are endonucleases cutting the RNA within, whilst RNase D is an trimming , exonuclease the ends of the tRNA molecules.
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