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you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100 micro moler reverse primer, 100 micromolar reverse primer, 200 micromole of each of the nucleotides, a sufficient amount of plymerase and bugges.
Is the amount of product dna in this set up limited by the amount of template dna, primers, or nucleotides?
Posted Date: 11/7/2012 1:12:50 AM | Location : United States
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