Tissue culture, Biology

Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png

Posted Date: 8/29/2012 6:15:20 AM | Location : United States







Related Discussions:- Tissue culture, Assignment Help, Ask Question on Tissue culture, Get Answer, Expert's Help, Tissue culture Discussions

Write discussion on Tissue culture
Your posts are moderated
Related Questions
Q. Pathophysiology of Cardiac tamponade? Progressive increase in pericardial fluid results in progressive increase in intrapericardial pressure, till a critical volume is reach


Structure and Content of Halsted Reitan battery Although there are several versions of the Halsted Reitan battery, the differences tend to be minor, and there appears to be a

Molecular Biology - transgenesis Difficult to successfully insert the gene then successfully re-implant embryo possible pleiotropic effects low success rate / tak

is a cyclone density dependent or independent


Define the Solar Concentration? As in food dehydration, one of the simplest methods of evaporating water is with solar energy. A typical example of this method is production a

What is Organelles? Organelles :   Eukaryotic cells contain various membrane-bound structures called organelles, in contrast to prokaryotes, which lack a definite internal or

Fertilization is the fusion of the two gametes (sperm and ovum) to produce a zygote which develops into the new individual with a genetic heritage derived from both the parents. S

Which of the following is an active transport process? A. Net flux of sodium across the plasma membrane via voltage-gated sodium channels. B. Net flux of glucose across the