The principle of dna cloning, Biology

Assignment Help:

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 


Related Discussions:- The principle of dna cloning

smog - london smog and photochemical smog, The word 'smog' consists of two...

The word 'smog' consists of two words 'smoke' and 'fog'. This name was given because Fog in the atmosphere Condense on the carbon particles of smoke to form smog. There are two typ

What is atrial extrasystoles, Q. What is Atrial Extrasystoles? Atrial p...

Q. What is Atrial Extrasystoles? Atrial premature beats often occur at lower workloads and have little significance. As exercise increases, they usually subside and may then re

Poultry and duck diseases-infectious laryngotracheitis (ilt), Infectious la...

Infectious laryngotracheitis (ILT) Infectious laryngotracheities, caused by fowl Gallid Herpesvirus 1 belonging to the family Herpesviridae, is a quickly spreading acute res

Explain the behavioural change communication, Explain the Behavioural chang...

Explain the Behavioural change communication? One of the reasons for the widespread prevalence of PEM in our country is ignorance due to illiteracy, particularly among the fema

Chromosomes, how many arrs are present in mataphasic telocentric chromosome...

how many arrs are present in mataphasic telocentric chromosomes

Determine pull-ups test for check the body strength, Determine Pull-ups tes...

Determine Pull-ups test for check the body strength? This test is also done to measure upper body strength or endurance by maximum number of pull-ups completed. Subject hangs f

Determine the width of attached gingiva at implant site, Width of Attached ...

Width of Attached Gingiva at Implant Site The zone of attached gingiva needs to be intact and of adequate width to protect (by separating) the tissue around the implant. If it

Explain concept of evolutionary tree, Q. What is an evolutionary tree? Is t...

Q. What is an evolutionary tree? Is there a precise evolutionary tree known by science that explains the emergence and origin of every type of living being? Evolutionary tree i

How dietary constituents affecting the calcium absorption, How dietary cons...

How dietary constituents affecting the calcium absorption? Several dietary constituents have an effect on calcium absorption. The differences in fractional absorption from diff

Which kind of chemical bond maintains the pairing, Q. Which kind of chemica...

Q. Which kind of chemical bond maintains the pairing of each chain in the DNA molecule? To form the DNA molecule, purine bases bind to pyrimidine bases by intermolecular bonds

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd