The principle of dna cloning, Biology

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 

Posted Date: 4/11/2013 5:20:27 AM | Location : United States







Related Discussions:- The principle of dna cloning, Assignment Help, Ask Question on The principle of dna cloning, Get Answer, Expert's Help, The principle of dna cloning Discussions

Write discussion on The principle of dna cloning
Your posts are moderated
Related Questions
What is the procedure of Gas Exchange in human body? Exchange of oxygen with the blood is a specialized process. If oxygen were carried in solution in blood plasma (the liquid

How is reproduction done in beings of the class Reptilia? These beings reproduce sexually by internal fecundation by means of copulation among male and female individuals. They

Occur in 10 per cent to 40 per cent of all native-valve IE, and complicates aortic IE more commonly than mitral or tricuspid IE. Periannular infection is of even greater concern wi

The  nitrogenase  complex  is  extremely  sensitive  to  inactivation  by  O2,  so  the enzyme  must be protected  from this reactive  substance.  During the root nodules of legumi

what is the differences between spherical symmetry and radial symmetry

Q. Prevention and cure of botulism involves? The prevention and cure of botulism involves: 1. Strict adherence to safe food-processing practices by the food industry 2.

Structure of HIV HIV is a spherical enveloped virus of about 90-120 nm diameter .In nucleocapsid is icosahedral. Its genome consists of a single stranded RNA filament segmented

Give the results of DistaE Dissection ? Results :  For acute dissection of different parts of aorta, the mortality is reported as 9 to 33 per cent. In the International Regis

A patient was complaining of frequent urination, excessive thirst and dehydration. His fasting glucose level was found to be normal. Name the disease and its cause. Describe

Q. What are some major differences of the vertebrate nervous systems comparing to invertebrates? In vertebrates the nervous system is well-characterized, having dorsal neural c