The principle of dna cloning, Biology

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 

Posted Date: 4/11/2013 5:20:27 AM | Location : United States







Related Discussions:- The principle of dna cloning, Assignment Help, Ask Question on The principle of dna cloning, Get Answer, Expert's Help, The principle of dna cloning Discussions

Write discussion on The principle of dna cloning
Your posts are moderated
Related Questions
Q. Illustrate the tyndallisation? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by 24 hour in

Q. What is continuous full thickness incision? 2. Continuous full - thickness incisions are indicated for a variety of situations outlined below. This type of incision may be u

Define the Reaction to Food Additives? Food additives such as preservatives, flavour enhancers and colouring agents have been linked to adverse food reactions. Additives implic

Q. Drugs and Insulin used in diabetes? When diet, exercise or even weight reduction do not improve the diabetic symptoms and blood sugar levels, the uses of hypoglycemia drugs

Are the arterial pulsations normal? Is the pulse volume normal or increased? Is there a discrepancy of pulsation in any of the four extremities? A careful evaluation of pulsati

Explain the various types of Protein Structure? Protein Structure :  The structure of proteins can be examined at four levels of increasing complexity, with the primary struc

Q. Are proteins with the same number of each dissimilar amino acid that forms them necessarily identical proteins? Even if many proteins have the same number of each different

Class Bivalvia Body in a bilohed mantle enclosed in a two-valved shell; head reduced; mouth with labial palps but no radula; foot wedge-shaped; plate-like gills; sexes separa

What is the physical experiment that show that the protoplasm has contractility?

BASIC PRINCIPLES OF EMERGENCY PSYCHIATRY:   Nurses handling psychiatric  emergencies should keep the following principles in mind:  The initial approach to a clienvpatien