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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Starch Retrogradation The starch paste or solution obtained after the gelatinisation is not stable and generally produces a viscoelastic, firm and rigid gel. Structural transf
D uck septicemia (duck pasteurellosis/new duck disease) The disease is caused by Pasteruella anatipestifer (Syn. Riemerella anatipestifer ) that mostly affects growing duck
what is respiratory system
Chikungunya Chikungunya, a metazoonosis, is caused by chikungunya virus belonging to genus Alphavirus of the family Togavirideae. The virus is transmitted by infected mosquito
How is synthesis of soluble proteins taking equatorial part on the surface of lens? Synthesis of soluble proteins taking equatorial part on the surface of lens as follows: 1
What is Glands? The central nervous system controls much of the endocrine system through the hypothalamus. The hypothalamus directs many of the body's functions through the "ma
Define Efficiency - terminologies used in chromatography? Efficiency in chromatography is expressed as number of theoretical plates. H = L/N Where, H is heigh
Explain Changing Body Composition with Ageing? With ageing, a progressive decline in the water content and the lean body mass is accompanied by an increasing proportion of body
Cellular Type - Endosperm In this type, as the name indicates, the division of the primary endosperm nucleus is immediately followed by the laying down of a wall, which is us
Byproducts high in energy and nitrogen Byproducts in this category include products such as blood meal and poultry offal meal, fish meal, extracted oilseed meals/cakes and wast
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