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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Explain the Metabolism - Zinc? Zinc has been found to play an important biological role in our body. Zinc ions can be chelated and precipitated by a number of chelating agents
Citric Acid Cycle The citric acid cycle, also known as tricarboxylic acid (TCA) cycle, lies at the heart of aerobic metabolism. it is involved in the breakdown of all three maj
Dinitrophenol, DNP, was prescribed in low doses in the 1940's to help obese patients lose weight. It's use for this purpose was discontinued when several users died. DNP makes the
what is nuclus
Human embryo: After fertilization the human embryo divides mitotically and develops two membranes: 1) Chorion 2) Amnion Functions of the membranes: i) Chorion : It
Maintenance sequences of implant Achieving a successful result with the treatment is only half the battle won. The main challenge is maintain that result over a long period of
Define Causes of iodine deficiency disorders? We have studied above that iodine deficiency is a naturally occurring ecological phenomenon that is present in many parts of the w
Monkey B virus disease The disease is caused by Herpesvirus simiae or B virus, a member of the herpes group of viruses. The infection occurs naturally among primates of the ge
Explain Vasopressindrug term? As a single bolus of 40 U, vasopressin has been recommended as an alternative to epinephrine in VF/pulseless VT refractory to defibrillation. Furt
Planning of Nursing Care Administer the drugs as prescribed - Prevent infection Control bleeding Implementation of Nursing Care Administer th
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