Somatic and germline gene therapy, Biology

All the cells present in the body contain genes and an approach targeted to modulate the expression of genes is known as gene therapy. This novel approach has emerged by the end of 20th century and comes up with a promise of treating a large number of genetic disorders by ultimately modifying the gene expression (Jin et al, 2008). The two forms of gene therapy are somatic and germ line.

Somatic gene therapy: During this therapeutic approach, the genetic diseases are treated by inserting recombinant genes into non-reproductive or somatic cells. This kind of approach is limited to the patient and does not get transmitted to the future generations of. A normal copy of the malfunctioning gene is inserted into the mutant cells due to which an improvement in the disease phenotype is observed (Dorin, 1996). In the recent past, this technique has been found to be successful in case of familial hypercholesterolaemia and adenosine deaminase deficiency. For treating these disorders hepatocytes and T cells have been used for gene transfer respectively (Dorin, 1996). It has also shown significant success in case of cystic fibrosis and cancer. Various vehicles, like viral and non viral vectors help in the introduction of the genetic material into the mutated cells (Elias and Annas, N.D). This therapeutic approach is categorized into three sections:

  • ex vivo - The genetic engineering of the cell is done outside the body and finally the cells are returned to the body. (Ex: blood cells)
  • in situ - The cells of the affected tissues are directly transfected with vectors carrying genetic material. (Ex. Tissues of the respiratory tract)
  • in vivo - The vector is directly injected into the blood stream and this method is still in the developmental stages (Anderson, N.D).

However this approach produces short term results as the tissues die and get replaced with other cells.

Posted Date: 8/9/2012 6:32:53 AM | Location : United States







Related Discussions:- Somatic and germline gene therapy, Assignment Help, Ask Question on Somatic and germline gene therapy, Get Answer, Expert's Help, Somatic and germline gene therapy Discussions

Write discussion on Somatic and germline gene therapy
Your posts are moderated
Related Questions
Photoreceptors Photoreceptors are concerned in absorption of light by photosensitive pigments. The chemical change involved produces the impulse concerned in the nerve cells.

What is the Expression of Moisture Content? Moisture content, you would realize, is expressed in one of two ways i.e dry weight basis (dwb) or wet weight bases (wwb). Moisture

Which of the following best defines the reasons why the sliding clamp is able to confer processivity on DNA polymerase and not DNA primase? A. The sliding clamp coats the templ

Calculate the pH: 300mL of 0.25M sodium ascorbate plus 150mL of 0.2M HCl (the pKa of ascorbic acid is 4.04) okay, what's the conjugate acid and base of this problem? Can someone wa

The doctor ordered 3 teaspoons of Basaljel suspension in water or juice. How many cc of Basaljel (aluminum carbonate) should the nurse place in the medicine cup?

(a) What carbohydrates does a plant make from glucose? (b) Which of these carbohydrates is transported round the plant? (c) Which carbohydrate is the main s

Chloroplast In plants, chloroplasts are the essential organelles for carrying out photosynthesis. Chloroplasts are found in all green plants. Chloroplast occurs mainly

Give the introduction of genesis of coronary artery diseases and risk factors? Cardio-vascular diseases have their roots in the structure and functional alterations in the vasc

Minerals :- Phosphates Food Source      Ubiquitous, animal products tend to be good sources Nutritional Functional role Essential nutrient: Deficiency is rare due

Q. What is folic acid? Why is the anemia caused by deficiency of folic acid known as megaloblastic anemia? The folic acid when ionized it is called folate is a coenzyme that pa